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A new enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against foot-and-mouth disease virus: II. Application
Affiliation:1. IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain;2. Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, 08003 Barcelona, Spain;3. Departament d''Agricultura, Ramaderia i Pesca (DARP), Generalitat de Catalunya, Spain;4. Departament de Ciència Animal, ETSEA, Universidad de Lleida, 25198, Spain;5. Centro de Biología molecular “Severo Ochoa” (CSIC-UAM), Cantoblanco, 28049 Madrid, Spain;1. University of Novi Sad, Faculty of Sciences, Department of Chemistry, Biochemistry and Environmental Protection, Trg D. Obradovića 3, Novi Sad 21000, Serbia;2. Division of Physical Science and Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand;1. Agricultural Research Council – Onderstepoort Veterinary Research, Private Bag X5, Onderstepoort, 0110, South Africa;2. Department of Veterinary Tropical Diseases, University of Pretoria, Private Bag X04, Onderstepoort, 0110, South Africa
Abstract:
The liquid-phase blocking sandwich ELISA has been evaluated for the serological study of antibodies against foot-and-mouth disease virus (FMDV). The titres recorded for sera from a population of more than 300 British uninfected, unvaccinated cattle which were examined against each of the seven immunologically distinct FMDV types were less than 1 in 40. alternative to the existing VN test for the quantification of antibodies against FMD virus.
Keywords:
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