新疆雪莲胚芽的组织培养和植株再生

目的:以新疆雪莲胚芽为外植体,建立一套新疆雪莲快速繁殖技术。方法:以MS培养基为基本培养基,通过研究植物生长物质BA和NAA对丛生芽的分化、丛生芽的增殖和生根的影响找出相应的最佳生长物质组合。结果:在附加NAA0.02-0.1mg·L^-1和BA2mg·L^-1的MS培养基上,所有的胚芽都可在45d内诱导出丛生芽;丛生芽切成小块后接入含NAA0.1-0.2mg·L^-1和BA2-3mg·L^-1的培养基中,都可在30d快速增殖;在含0.4mg·L^-1NAA的大量元素减半的MS培养基中,92%的不定芽可在25d内生根;再生植株移栽后,生长较好,成活率达70%。结论:新疆雪莲的胚芽可通过不定芽途径高频率的形成植株。

Tissue culture and plantlet regeneration from embryo of Saussurea involucrate

Objective: To establish a protocol of rapid clonal propagation of Saussurea involucrate by using embryo as explants. Method: MS medium was used as basal medium and BA and NAA were supplemented to find out the optimal hormone combinations for adventitious buds initiation, adventitious bud multiplication and rooting. Result: All embryo explants started to grow adventitious buds within 45 days when they were cultured on the media supplemented with 0.02-0.1 mg·L^-1 NAA and 2 mg·L^-1 BA; The adventitious buds multiplicated within 30 days when they were transferred to the media containing 0.1-0.2 mg·L^-1 NAA and 2-3 mg·L^-1 BA; 92% of the adventitious buds rooted well after they were planted on the MS medium containing macroelements at half strength and 0.4 mg·L^-1 NAA for 25 days. The regenerated plantlets grew well after they were transplanted and the survival rate was up to 70% . Conclusion: Plantlets of S. invducrate regenerated high frequence through adventitious bud.