LDL-C直接测定法在生化分析仪上的应用

用日本第一化学药品株式会社LDL—C试剂盒在BeckmanCX4型自动生化分析仪上直接测定血清LDL—C含量。方法批内CV 0 37%～ 1 11% ,批间CV为 2 11%～ 3 0 2 % (n =2 0 )。平均回收率为 98 9% ,在 8 10mmol/L浓度范围内呈良好线性关系。TG <5 0 0mmol/L时与聚乙烯硫酸沉淀法 (X1)及Friedewald(X2 )公式计算结果相关良好 ,回归方程分别为Y =0 988X1+0 0 47,r=0 95 1,Y =0 96 1X2 +0 0 5 2 ,r=0 930。LDL—C直接测定法简单、快速 ,用血量少 ,勿需沉淀分离 ,适宜于自动化分析。

The application of direct method for measuring low-density lipoprotein cholesterol in biochemistry autoanalyzer

The reagent kit of direct method for mearsuring low density lipoprotein cholesterol(LDL-C)based on the principle of select inbibition and Beckman CX7 biochemistry autoanalyzer was used to determin the level of serum LDL-C.The within run CV and between run CV were 0 37～1 11%,2 11～3 02%(n=20)respectively.The average recovery was linear up to 8 10mmol/L.In case of TG<5 0mmol/L,the directly determined LDL-C values (Y)closely correlated with those obtained by precipitation with polyvinyl sulfate(x 1)(Y=0 988x 1+0 047,r=0 951)and Friedewald formula(x 2)(Y=0 961x 2+0 052,r=0 930).The conclusion of this article was:the direct method for measuring LDL-C is simple,rapid,unnecessary for the precipitative centrifugue,and adapted to full autoassay.