乳腺癌患者免疫指标检测及其临床意义

目的:分析乳腺癌及乳腺良性疾病患者机体免疫指标的特点及临床意义。方法:应用流式细胞术对30例乳腺癌患者、45例乳腺良性疾病患者外周血T细胞亚群及NK细胞进行检测。结果:乳腺癌患者CD3 、CD4 细胞百分比显著低于乳腺良性疾病组(P<0.05);CD8 细胞百分比以及CD4 /CD8 比值在乳腺癌组和乳腺良性疾病组之间无显著差异(P>0.05);乳腺癌患者NK细胞百分比显著高于乳腺良性疾病组(P<0.05);乳腺癌腋淋巴结转移组CD3 细胞百分比及CD4 /CD8 比值明显低于腋淋巴结无转移组(P<0.05),而NK细胞百分比显著高于腋淋巴结无转移组(P<0.05)。结论:乳腺癌患者存在细胞免疫功能紊乱,检测外周血T淋巴细胞亚群表达可能对评估患者的细胞免疫功能及疾病的预后具有一定意义。

Detection of anti-tumor immunity of patients with breast cancer and its clinical significance

Objective:To analyze the anti-tumor immunity of patients with breast cancer and breast benign disease and its clinical significance.Methods:T-lymphocyte subsets and NK cells in peripheral blood from 30 patients with breast cancer and 45 patients with breast benign disease were determined by flow cytometry(FCM).Results:Percentages of CD3~ and CD4~ cells in patients with breast cancer were significantly lower than those with breast benign disease(P<0.05).There was no significant difference in percentage of CD8~ cells and CD4~ /CD8~ ratio between the patients with breast benign disease and those with breast cancer(P>0.05).The level of NK cells was significantly increased in patients with breast cancer(P<0.05).The percentage of CD3~ cells and CD4~ /CD8~ ratio in patients with axillary node-positive breast cancer were remarkably lower than those with axillary node-negative breast cancer(P<0.05),but the level of NK cells was remarkably higher(P<0.05).Conclusion:The cell immunity is abnormal in patients with breast cancer.Detection of T lymphocyte subsets in peripheral blood has clinical significance in evaluation of the immunity and prognosis of patients with breast cancer.