| 大鼠脊髓横断损伤后CNTF mRNA的表达变化 |
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| 引用本文： | 赵彩霞,刘佳,章为.大鼠脊髓横断损伤后CNTF mRNA的表达变化[J].四川大学学报(医学版),2009,40(3). |
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| 作者姓名： | 赵彩霞 刘佳 章为 |
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| 作者单位： | 四川大学华西基础医学与法医学院,组织胚胎学与神经生物学教研室,成都,610041 |
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| 基金项目： | 国家自然科学基金，纽约中华医学基金会资助项目 |
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| 摘 要： | 目的 观察睫状神经营养因子(CNTF)mRNA在脊髓全横断大鼠损伤后的表达变化,探讨CNTF在脊髓损伤修复中的作用.方法 40只SD大鼠,随机分为假手术组和脊髓损伤组(SCI组),后者根据取材时间又分为术后1 d、3 d、7 d和14 d组.SCI组动物选择在T10节段全横断脊髓,假手术组除不横断脊髓外其余步骤与SCI组相同.动物处死前进行BBB评分评估后肢运动功能的变化.取手术部位头端和尾端0.5 cm脊髓,采用RT-PCR技术检测CNTF mRNA在各组脊髓中的表达变化.结果 行为学评分随着损伤时间的推移呈小幅度增加,但后肢无运动功能恢复;术后1 d组和3 d组横断点尾端脊髓中CNTF mRNA的表达与假手术组相比上调(P<0.05).而7 d组和14 d组的表达量又逐渐恢复正常,与假手术组比较差异无统计学意义.结论 脊髓损伤早期CNTF的表达变化可能与脊髓损伤修复有关.
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| 关 键 词： | 睫状神经营养因子 脊髓损伤 逆转录-聚合酶链反应(RT-PCR) BBB评分 |
Expression of Ciliary Neurotrophic Factor mRNA in Spinal Cords of Adult Rats with Transection Injury |
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| ZHAO Cai-xia,LIU Jia,ZHANG Wei.Expression of Ciliary Neurotrophic Factor mRNA in Spinal Cords of Adult Rats with Transection Injury[J].Journal of West China University of Medical Sciences,2009,40(3). |
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| Authors: | ZHAO Cai-xia LIU Jia ZHANG Wei |
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| Institution: | ZHAO Cai-xia,LIU Jia,ZHANG Wei.Department of Histology,Embryology , Neurobiology,West China School of Preclinical , Forensic Medicine,Sichuan University,Chengdu 610041,China |
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| Abstract: | Objective To determine the expression of ciliary neurotrophic factor(CNTF)mRNA in transactional injured spinal cords and the role of CNTF in repairing and regenerating spinal cords in adult rats.MethodsForty clean adult Sprague Dawley(SD)rats were randomLy divided into sham group and operation group(SCI group).The SCI group was subdivided into four groups,with the rats sacrificed 1,3,7 and 14 days after the operations(day of postoperation,DPO),respectively.The spinal cords of the rats were completely transe... |
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