RP-HPLC同时测定穿山龙药材中原薯蓣皂苷、甲基原薯蓣皂苷和伪原薯蓣皂苷的含量

目的:建立测定产地黑龙江大兴安岭穿山龙供试品中水溶性皂苷含量的方法.方法:采用RP-HPLC,Diamonsil (钻石)C18色谱柱(4.6 mm×250 mm,5 μm),流动相乙腈-水梯度洗脱,检测波长203 nm,柱温30℃.结果:原薯蓣皂苷浓度在50 ～ 500 mg·L-1、甲基原薯蓣皂苷在35 ～350 mg·L-1、伪原薯蓣皂苷质量浓度在15 ～ 150 mg·L-1与峰面积呈良好的线性关系,r=0.9999(n =6).方法学考察中各项实验结果的RSD均＜3％.结论:方法重复性好,具有良好的回收率,稳定性佳,为穿山龙药材的质量控制提供了参考.

Simultaneous Determination the Contents of Protodioscin, Methylprotodioscin, Pseudoprotodiosc in Rhizoma Dioscoreae Nipponicae with RP-HPLC

Objective:Establish a method for determining the content of water soluble saponins of Rhizoma Dioscoreae Nipponicae which grow in Daxinganling area of Heilongjiang Province.Method:RP HPLC was used on an Diamonsil C18 column(4.6 mm×250 mm,5 μm) with acetomitrile-water as mobile phase by gradient elution for the separation and determination of saponins of Rhizoma Dioscoreae Nipponicae,the detection wavelength was at 203 nm,column temperature 30 ℃.Result:The linear ranges were 50-500 mg·L-1 for protodioscin concentration,35-350 mg·L-1 for methyl protodioscin concentration,15-150 mg·L-1 for pseudoprotodioscin concentration,the calibration curve of each solvent had good linear relationship within the range with a correlation coefficient of 0.999 9(n=6).On the basis of methodology of the test results show RSD <3%.Conclusion:All the results show that the method has good reproducibility,recovery rate and stability.This has provided a basis for Rhizoma Dioscoreae Nipponicae quality control.