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| 黏着斑激酶对肝癌细胞生物学行为的影响 | |
| 引用本文： | 方祛,方新初,王丽影,涂立宇,查锡良. 黏着斑激酶对肝癌细胞生物学行为的影响[J]. 中华肿瘤杂志, 2001, 23(2): 125-127 |
| 作者姓名： | 方祛 方新初 王丽影 涂立宇 查锡良 |
| 作者单位： | 1. 上海医科大学生化教研室卫生部糖复合物重点实验室 2. 上海医市高等医学专科学校 |
| 基金项目： | 国家自然科学基金资助项目（39700169）；上海市科委基金资助项目(00JC14042) |
| 摘 要： | 目的：探讨降低黏着斑激酶（FAK）的表达对FAK高表达的人肝癌细胞株SMMC－7721恶性生物学行为影响。方法用Western杂交法比较不同肝癌细胞株FAK含量的差别，构建FAK反义质粒，转染至FAK高表达的细胞株，研究其多种细胞生物学行为的变化。结果SMMC－7721FAK含量比正常人肝细胞L02明显增高，转染FAK反义质粒后、SMMC－7721细胞生长受到抑制，软琼脂培养克隆形成率下降；细胞周期分析，S期细胞下降15％；黏附能力下降；细胞表面整连蛋白含量不变。结论在SMMC－7721中存在FAK过表达，降低FAK表达能部分逆转该肝癌细胞株的恶性表型。 |
| 关 键 词： | 肝肿瘤 肝细胞癌 黏着斑激酶 基因转染 细胞生物 |
| 修稿时间： | 1999-07-30 |
Influence of focal adhesion kinase(FAK) on the biological behaviors of human hep atocellular carcinoma cells |
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| Y Fang,X Fang,L Wang. Influence of focal adhesion kinase(FAK) on the biological behaviors of human hep atocellular carcinoma cells[J]. Chinese Journal of Oncology, 2001, 23(2): 125-127 | |
| Authors: | Y Fang X Fang L Wang |
| Affiliation: | Department of Biochemistry, Ministry of Public Health Key Laboratory of Glycoconjugate Research, Shanghai Medical University, Shanghai 200032, China. |
| Abstract: | OBJECTIVE: To study the effect of reducing FAK expression on the malignant biological behaviors of FAK-overexpressed hepatocellular carcinoma cell line SMMC-7721. METHODS: FAK expression of different hepatocelluar carcinoma cell lines was detected by Western blot analysis. Antisense plasmid of FAK was constructed and transfected into SMMC-7721. RESULTS: SMMC-7721 expressed much more FAK than did hepatocyte line L02. When transfected with autisense FAK, it showed reduced growth rate and colony formation in soft agar, and the number of cells in S phase was reduced by 15%. The adhesion ability of antisense FAK-transfeected cells was also decreased with no change of integrin expression. CONCLUSION: FAK is overexpressed in the human hepatocellular carcinoma cell line SMMC-7721. Reducing the FAK expression can partly reverse the malignant behaviors of the cell line. |
| Keywords: | Liver neoplasms Carcinoma hepatocellular Focal adhesion kinase Gene transfection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |