实验性牙周炎动物模型牙龈组织一氧化氮含量检测与分析

目的检测分析大鼠实验性牙周炎动物模型牙龈组织中一氧化氮（NO）的含量，初步探讨NO在牙周炎发生和发展中的作用。方法建立大鼠实验性牙周炎动物模型，采用酶法检测牙龈组织中NO3^-和NO3^-的浓度，从而间接反映NO的含量。结果正常对照组、牙龈炎组及牙周炎组牙龈组织NO含量均存在显著性差异性（P〈0．01）。实验组1周、4周、7周和11周牙龈组织中NO含量存在显著性差异性（P〈0．01），NO含量随牙周炎病程的发展呈先降低后升高的趋势，并且牙龈组织NO含量还与牙槽骨的吸收有关。结论NO在牙周炎的病理过程中对于疾病的发生和发展起到了重要的作用。

Detection and Analysis of Nitric Oxide Level in Gingival Tissue in Experimental Animal Model of Periodontitis

Objective To investigate the role of nitric oxide(NO)in the pathogenesis of periodontal diseases by detecting and analyzing NO level in gingival tissue in experimental animal model of periodontitis.Methods Experimental animal model of periodontitis in rats was built.Then NO2-and NO3-concentrations,which reflect NO level indirectly,were measured by NO kit in gingival tissue of rat with experimental periodontitis.Results The significant differences of NO level in gingival tissue were found among normal group,gingivitis group and periodontitis group(P<0.01).In experimental groups,the NO concentrations were significantly different at 1w,4w,7w and 11w,and displayed a curve during the development of periodontal diseases.They were also related to alveolar bone loss.Conclusions NO plays an important role in the pathophysiology of periodoitis,as a prompting mechanism to the disease onset and progression.