川芎嗪对局灶性脑缺血再灌注后Bcl-2、Bax蛋白表达的影响

目的：探讨川芎嗪对大鼠局灶性脑缺血再灌注后脑组织Bcl-2和Bax蛋白表达的影响。方法：SD大鼠18只随机分为3组各6只：A组为假手术组，B组为缺血再灌注组，C组为川芎嗪干预组。采用免疫组化与医学图像分析检测大鼠大脑皮层Bcl-2和Bax蛋白阳性细胞的数目、平均灰度。结果：B组与A组比较损伤侧脑组织Bcl-2、Bax蛋白阳性细胞数目均增多，平均灰度均降低（P〈0．01）；C组与B组比较Bcl-2蛋白阳性细胞数目增多，平均灰度降低，Bax蛋白阳性细胞数目减少，平均灰度升高（P〈0．05）。结论：局灶性脑缺血再灌注后Bcl-2、Bax蛋白表达增强；川芎嗪可上调Bcl-2蛋白及下调Bax蛋白的表达，这可能是川芎嗪治疗缺血性脑血管病的机制之一。

Effects of Ligustrazine on the Expression of Bcl-2 and Bax Proteins after Focal Cerebral Ischemia and Reperfusion

Objective:To investigate the effects of ligustrazine(LZ) on the expression of Bcl-2 and Bax proteins after focal cerebral ischemia and reperfusion in rats' brains.Methods: Eighteen SD rats were randomly divided into three groups.Group A: sham-operated group(n=6);group B: ischemia and reperfusion group((n=)6);group C: LZ intervention group(n=6).The immunohistochemistry and MIPS were used to measure the number and mean grey level of Bcl-2 or Bax positive cells in cerebral cortex of the three groups.Results: In ischemic side of group B,the number of Bcl-2 or Bax positive cells was higher and mean grey level was lower than those in sham-operated side of group A(P<0.01).In ischemic sides,compared with group B,the number of Bcl-2 positive cells in group C was increased and mean grey level was decreased;while the number of Bax positive cells in group C was reduced and mean grey level was elevated(P<0.05).Conclusion: The expression of Bcl-2 or Bax protein was increased after focal cerebral ischemia and reperfusion.LZ could up-regulate the expression of Bcl-2 protein and down-regulate the expression of Bax protein at the same time,which might be one of mechanisms of LZ treating ischemic stroke.