DADS下调Cathepsin D抑制人胃癌MGC803细胞侵袭转移

目的研究二烯丙基二硫（ DADS ）是否通过下调组织蛋白酶D （ Cathepsin D ）的表达抑制人胃癌MGC803细胞侵袭转移。方法 RT-PCR和Western blot检测DADS及干扰Cath-D对人胃癌MGC803细胞Cath-D mRNA与蛋白表达变化；细胞划痕实验和Transwell实验分别检测DADS及干扰Cath-D对人胃癌MGC803细胞侵袭转移能力的变化。结果 DADS作用人胃癌MGC803细胞24 h后,Cath-D mRNA与蛋白表达水平明显下降（ P〈0.05）,划痕距离明显增宽（P〈0.05）,穿膜细胞数明显减少（P〈0.05）；siRNA干扰Cath-D后,Cath-D mRNA与蛋白的表达水平明显下降（P〈0.05）,划痕距离明显增宽（P〈0.05）,穿膜细胞数明显减少（P〈0.05）。结论DADS能抑制人胃癌MGC803细胞的侵袭转移能力,其机制可能与下调Cath-D 的表达有关。

Down-regulated Cathepsin D Inhibits Migration and Invasion in HumanGastric Cancer MGC803 Cells Induced by Diallyl Disulfide

ObjectiveTo investigate whether diallyl disulfide (DADS) inhibits human gastric carcinoma MGC803 cells invasion and metastasis by down-regulating the expression of Cathepsin D.MethodsThe mRNA and protein expression level of Cath-D were detected by RT-PCR and Western blot respectively;Migration and invasion potentials were examined by Wound Healing assay and transwell assay.ResultsHuman gastric carcinoma MGC803 cells exposed to DADS30 mg/L for 24h,the mRNA and protein expression level of Cath-D were significantly decreased (P<0.05),scar distance was widened (P<0.05),the number of cancer cells which passed through the Matrigel coated membrane was reduced (P<0.05);after Cath-D was interfered by siRNA,the mRNA and protein expression level of Cath-D were significantly decreased (P<0.05),scar distance was widened (P<0.05),the number of cancer cells which passed through the Matrigel coated membrane was reduced (P<0.05).ConclusionsDADS can significantly inhibit the ability of migration and invasion ability of human gastric carcinoma MGC803 cells and its mechanism may be related to the expression of down-regulating Cath-D.