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| 西藏红雪茶多糖含量测定 | |
| 引用本文： | 庄筱葳,刘秀芳,毛贵元,陈本超,蔡光明,夏新华. 西藏红雪茶多糖含量测定[J]. 中国实验方剂学杂志, 2012, 18(4): 103-106 |
| 作者姓名： | 庄筱葳 刘秀芳 毛贵元 陈本超 蔡光明 夏新华 |
| 作者单位： | 1. 解放军302医院中药研究所,北京 100039;湖南中医药大学药学院,长沙 410208 2. 解放军302医院中药研究所,北京 ,100039 3. 湖南中医药大学药学院,长沙 ,410208 |
| 摘 要： | 目的:研究西藏红雪茶多糖含量测定的方法.方法:采用苯酚-硫酸法测定总糖含量,3,5-二硝基水杨酸(DNS)法测定还原糖含量,两者的差值即为多糖含量.结果:总糖标准曲线方程为OD =0.051 4C- 0.0146(r=0.999 2),还原糖标准曲线方程为OD =0.022 9C-0.086(r =0.999 9),两者分别在2.5 ～12.5,8～40 mg·L-1有良好的线性关系.测得LC,LCA,LCP多糖含量分别为63.6％,82.6％,57.6％.结论:该方法操作快速、简便、灵敏、准确、重复性好. |
| 关 键 词： | 红雪茶 总糖 还原糖 苯酚-硫酸法 3,S-二硝基水杨酸法 |
Determination of Lethariella cladonioides Polysaccharide from Tibet |
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| ZHUANG Xiao-wei,LIU Xiu-fang,MAO Gui-yuan,CHEN Ben-chao,CAI Guang-ming and XIA Xin-hua. Determination of Lethariella cladonioides Polysaccharide from Tibet[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2012, 18(4): 103-106 | |
| Authors: | ZHUANG Xiao-wei LIU Xiu-fang MAO Gui-yuan CHEN Ben-chao CAI Guang-ming XIA Xin-hua |
| Affiliation: | Institute of Chinese medicine, 302 Military Hospital of China, Beijing 100039,China; College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208,China;Institute of Chinese medicine, 302 Military Hospital of China, Beijing 100039,China;Institute of Chinese medicine, 302 Military Hospital of China, Beijing 100039,China;Institute of Chinese medicine, 302 Military Hospital of China, Beijing 100039,China; College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208,China;College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208,China;Institute of Chinese medicine, 302 Military Hospital of China, Beijing 100039,China |
| Abstract: | Objective: To establish a method for the determination of polysaccharide in Lethariella cladonioides. Method: Using microplate reader, the content of total sugar was determined by means of phenol-sulfuric acid method and 3,5-dinitro salicylic acid (DNS) method was used for the determination of reducing sugar, the difference between the two was the polysaccharide content. Results: The total sugar standard curve equation was OD=0.051 4C-0.014 6(r=0.999 2) and reducing sugar standard curve equation was OD=0.022 9C-0.086 (r=0.999 9). Respectively the linear range was 2.5-12.5 mg·L-1 and 8-40 mg·L-1. The polysaccharide content of LC, LCA, LCP were 63.6%, 82.6%, 57.6%. Conclusion :The method is fast, simple, sensitive, accurate and reproducible. |
| Keywords: | Lethariella cladonioides total polysaccharide reducing sugar phenol-sulfuric acid method DNS method |
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