桂苓神术汤及其有效部位的利尿作用

目的:研究桂苓神术汤的利尿作用,筛选利尿作用的有效部位并探讨其作用机制.方法:SD大鼠,随机分为18组:正常组、模型组,呋噻米组,桂苓神术汤及石油醚部位、乙酸乙酯部位、正丁醇部位、剩余部位分别设10.0,5.0,2.5 g·kg-13个剂量组.每组大鼠均按40 mL· kg-生理盐水ig,形成盐水负荷模型.45 min后,ig给予不同剂量的桂苓神术汤及不同极性部位,用大鼠代谢笼法观察大鼠6h的尿量,并测定尿液pH及Na+,K+,Cl-的浓度.结果:桂苓神术汤组(5.0,2.5 g·kg-1),乙酸乙酯部位组(5.0 g·kg-1),剩余部位组(5.0 g·kg-1)均能显著增加盐水负荷大鼠6h的尿量；尿中K+排出量较模型组均显著升高,分别为(850.3±78.8),(816.6±91.1),(826.3±84.9),( 776.7±69.1)mmol·L -1,Na+,C1-排出量较模型组无显著差异.结论:桂苓神术汤及有效部位具有缓和持久的利尿作用,其利尿作用机制可能为增加大鼠尿液中K+的排泄.

Diuetic Effects of Guiling Shenzhu Decoction and its Different Effective Parts

Objective: To study the diuetic effects and its mechanism of Guiling Shenzhu decoction and its different effective parts. Method: The effect on urinre volum and urinary electrolyte (Na⁺, K⁺, Cl^-) were assessed by using rat metabolic cage and electrolyte analyzer following oral administration of Guiling Shenzhu decoction (10.0, 5.0, 2.5 g·kg^-1) and its different polar fractions (10, 5.0, 2.5 g·kg^-1). Result: Guiling Shenzhu decoction (5.0, 2.5 g·kg^-1) and extraction of ethyl acetate (5.0 g·kg^-1) and residual water layer (5.0 g·kg^-1) had good diuretic effect. The urine K⁺ excretion increased significantly, but Na⁺ and Cl^- excretion did not increase. Conclusion: Guiling Shenzhu decoction and extraction of ethyl acetate and residual water layer have significant and persistent diuretic effect. They cause marked increase in urinary K⁺level and it may be the diuetic mechanism.