款冬花及其总生物碱的肝脏毒性

目的: 对款冬花的肝脏毒性进行初步的研究探讨。 方法: 昆明种小鼠 ig 款冬花水煎液生药20,40 g·kg^-1连续4周,测定血清丙氨酸氨基转移酶(ALT),门冬氨酸氨基转移酶(AST)活性,肝组织称重,计算肝脏系数,观察肝脏病变情况并评分;利用精密肝切片培养技术,将款冬花水煎液0.005,0.05 g·L^-1和总生物碱0.5,2.0 g·L^-1与肝切片分别共同培养24,6 h后,制备肝组织匀浆, BCA法测定匀浆液中蛋白含量,连续监测法测定并计算每微克蛋白中ALT,AST,乳酸脱氢酶(LDH),γ-谷氨酰胺转移酶(GGT)的漏出率。 结果: 款冬花水煎液40 g·kg^-1ig 4周后,镜下观察雌性动物款冬花水煎液组与对照组比较有明显病理改变,且伴有肝脏系数的明显增加;款冬花水煎液及总生物碱与肝切片共培养后,水煎液2个剂量组均能引起ALT漏出率的显著升高;总生物碱0.5 g·L^-1组能引起肝切片LDH,ALT漏出率显著升高,2.0 g·L^-1组能引起肝切片GGT漏出率显著升高,蛋白含量显著下降。 结论: 款冬花体内体外试验均显示出一定的肝脏毒性。

Hepatotoxicity on Water Extracts and the Total Alkaloid of Farfarae Flos

Objective: To study the hepatotoxicity of Farfarae Flos. Method: KM mice were given the water extracts of Farfarae Flos orally in 20, 40 g·kg^-1 doses for successive 4 weeks, enzyme activity of aspartate transaminase(AST), alanine transaminase(ALT) in serum of mice were measured, coefficient of liver were calculated, and liver histopathology examination were conducted. Using the precision-cut slice technology, culture liver slices for 24 hours with water extracts in concentration 0.005, 0.05 g·L^-1 and 6 hours with the total alkaloid of Farfarae Flos in concentration 0.5, 2.0 g·L^-1, the slice homogenate were prepared, protein concentration were detected by BCA protein assay method, enzyme activity of ALT, AST, lactate dehydrogenase(LDH), r-glutamgl thanspeplidase(GGT) were detected by enzyme kinetics method and leakage were calculated. Result: The water extract of Farfarae Flos 40 g·kg^-1 dose group of female had significant histopathology changes and organ coefficient of liver increased compared with control group. After co-culture for 24 hours with water extracts of Farfarae Flos , ALT leakage were significantly increased in two groups; LDH and ALT leakage were significantly increased with final concentration 0.5 g·L^-1, GGT leakage was significantly increased and protein content was obviously decreased with final concentration 2.0 g·L^-1 after co-culture for 6 hours with the total alkaloid of Farfarae Flos . Conclusion: Farfarae Flos displayed liver toxicity in some degree for in vivo and in vitro experiments.