五味子乙素联合泼尼松调节PI3K/Akt信号通路改善大鼠膜性肾病研究

目的 研究五味子乙素联合泼尼松对膜性肾病大鼠的作用及对其对PI3K/Akt信号通路的影响。方法 取15只SD大鼠作为对照组，60只大鼠采用阳离子化牛血清白蛋白（C-BSA）建立膜性肾病模型，造模完成后的大鼠随机分为模型组、泼尼松（2 mg/kg）组、五味子乙素（30 mg/kg）组、五味子乙素（30 mg/kg） ＋泼尼松（2 mg/kg）组。连续给药28 d后，分别测定各组大鼠24 h尿蛋白量、血清总胆固醇（TC）、三酰甘油（TG）、丙二醛（MDA）、超氧化物歧化酶（SOD）、尿素氮（BUN）及肌酐（Scr）水平，苏木精-伊红染色法进行组织病理学检查，Western blotting法检测大鼠肾脏p-Akt、Akt、PI3K-P85、PI3K-P110蛋白表达水平。结果 与模型组比较，泼尼松组、五味子乙素组、五味子乙素＋泼尼松组大鼠24 h尿蛋白量和血清TC、TG、MDA、BUN及Scr水平均显著降低（P<0.05），SOD水平显著升高（P<0.05），组织病理学明显改善，肾脏p-Akt、Akt、PI3K-P85、PI3K-P110蛋白表达水平均显著降低（P<0.05），且五味子乙素＋泼尼松组各项指标均优于单给药组。结论 五味子乙素联合泼尼松对大鼠膜性肾病发挥显著改善作用，作用优于单给药，其机制可能与调节PI3K/Akt信号通路有关。

Effect of schisandrin B combined with glucocorticoid on PI3K/Akt signal pathway in rats with membranous nephropathy

Objective To study the effect of schisandrin B combined with glucocorticoid on membranous nephropathy in rats and the effect of PI3K/Akt signal pathway in vivo. Methods Tatolly 15 SD rats were selected as the control group, and 60 rats were used to establish membranous nephropathy model with cationic bovine serum albumin (C-BSA). After modeling, the rats were randomly divided into model group, prednisone (2 mg/kg) group, schisandrin B (30 mg/kg) group, schisandrin B (30 mg/kg) + prednisone (2 mg/kg) group. After 28 days of continuous administration, 24-hour urinary protein, serum total cholesterol (TC), triglyceride (TG), malondialdehyde (MDA), total superoxide dismutase (SOD), urea nitrogen (BUN) and creatinine (Scr) were measured. Hematoxylin-eosin staining was used for histopathological examination, Western blotting was used to detect the protein levels of pAkt, Akt, PI3K-P85 and PI3K-P110 in kidney. Results Compared with model group, the levels of 24-hour urinary protein and serum TC, TG, MDA, BUN and Scr in prednisone group, schisandrin B group and schisandrin B + prednisone group were significantly lower than those in model group (P < 0.05). The levels of p-Akt, Akt, PI3K-P85 and PI3K-P110 in kidney were significantly decreased in prednisone group, schisandrin B group and schisandrin B + prednisone group (P < 0.05), and the effect of schisandrin B combined with prednisone group was better than that in prednisone and schisandrin B group. Conclusion Schisandrin B combined with prednisone can significantly improve membranous nephropathy in rats. The mechanism may be related to the regulation of PI3K/Akt signal pathway.