| 高效液相色谱法测定利咽颗粒中绿原酸的含量 |
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| 引用本文： | 蔡中齐,吴一天. 高效液相色谱法测定利咽颗粒中绿原酸的含量[J]. 中国中医药信息杂志, 2009, 16(8): 48-49 |
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| 作者姓名： | 蔡中齐 吴一天 |
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| 作者单位： | 1. 浙江医药高等专科学校,浙江,宁波,315100
2. 浙江省食品药品监督管理局,浙江,杭州,310012 |
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| 摘 要： | 目的建立高效液相色谱法测定利咽颗粒中绿原酸含量的方法。方法色谱柱为Diamondsil C18（4．6mm×150mm，5um），流动相为乙腈-0．4％磷酸（13：87），检测波长327nm，流速1．0mL／min。结果线性范围为0．026-0．26μg（r=0．9999），平均回收率为101．84％，RSI）=0．48％（n=6）。结论该法简便、准确、稳定，可用于利咽颗粒的质量控制。
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| 关 键 词： | 高效液相色谱法 利咽颗粒 绿原酸 含量测定 |
Determination of Chlorogenic Acid in Liyan Granule by HPLC |
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| CAI Zhong-qi,WU Yi-tian. Determination of Chlorogenic Acid in Liyan Granule by HPLC[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2009, 16(8): 48-49 |
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| Authors: | CAI Zhong-qi WU Yi-tian |
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| Affiliation: | CAI Zhong-qi, WU Yi-tian (1.ZhefiangPharmaceutical College, Ningbo 315100, China; 2.Zhejiang Food and Drug Administration, Hangzhou 310012, China) |
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| Abstract: | Objective To establish a HPLC method for determination of Chlorogenic acid in Liyan Granule.Method The determination was performed on Diamondsil C18 （4.6 mm× 150 mm, 5 μm） with mobile phase consisted of acetonitrile-0.4% phosphonic acid solution （13 ： 87） at a flow rate of 1.0 mL/min. The detective wavelength was set at 327 nm. Result The linear range of Chlorogenic acid was 0.026-0.26 μg （r=0.999 9） and the average recovery rate of Chlorogenic acid was 101.84% （RSD=0.48%, n =6）. Conclusion This method is simple, accurate and with good stabilization, and suitable for the quality control of Liyan gramule. |
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| Keywords: | HPLC Liyan Granule Chlorogenic acid content determination |
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