血清脂蛋白亚类和高密度脂蛋白胆固醇酯化速率的生物学变异

目的 研究血清HDL亚类(HDL2-C、HDL3-C)、LDL亚类(LDLa-C、LDLb-C)、FERHDL和MERHDL的个体内和个体间的生物学变异(CVI、CVG).方法 选取2010年9-10月卫生部北京医院20名健康成年志愿者,男女各10名.每隔2周采集空腹静脉血1次,共采集4次,用超速离心-高效液相色谱(HPLC)法测定各脂蛋白亚类水平及FERHDL和MERHDL,计算其CVI和CVG,并给出各项指标的分析质量要求.结果 不同血脂指标的生物学变异不同,HDL3-C和HDL2-C的平均CVI分别为5.5％和7.2％,但两者的CVG差别较大,分别为8.7％和45.5％；LDLa-C和LDLb-C平均CVI分别为11.2％和18.7％；FERHDL和MERHDL的CVI分别为11.9％和12.3％,其中FERHDL的CVG (49.5％)大于MERHDL的CVG(30.6％).CVI存在较大个体差异.结论 本研究考察了血清脂蛋白亚类和HDL胆固醇酯化速率的生物学变异,可为这些血脂类危险因素的分析质量控制及心血管病危险分析奠定基础.

Biological variations of serum lipoprotein subfractions and high-density lipoprotein cholesterol esterification rate

Objective To investigate the biological variations ( CVI,CVG ) of serum high-density (HDL2-C,HDL3-C ) and low-density (LDLa-C,LDLb-C ) lipoprotein subfractions and high-density lipoprotein cholesterol esterification rates (FERHDL and MERHDL ).Methods Twenty healthy volunteers,10 males and 10 females,were recruited for this study from September to October,2010.Blood was collected four times from each individual with a 2-week interval between each sampling.Serum lipoprotein subfraction cholesterol levels were measured by ultracentrifugation/HPLC,FERHDL and MERHDL were measured by HPLC.Within-subject ( CVI) and between-subject ( CVG.) biological variations and quality specifications for precision,bias and total error were calculated.Results The average CVI of this group were 5.5％ and 7.2％ for HDL3-C and HDL2-C,11.2％ and 18.7％ for LDLa-C and LDLb-C,11.95％ and 12.3％ for FERHDL and MERHDL,respectively.The CVG for HDL2-C was 45.5％,much higher than that of HDL3-C (8.7％),and FERHDL(49.5％ ) had a higher CVG than MERHDL (30.6％ ).For each analyte,there was a considerable variation of CVI among individuals.Conclusions Biological variations of lipoprotein subfractions,FERHDI and MERHDL have been estimated.These rsults will play an important role in quality specifications and cardiovascular disease risk assessment.