| 真菌Polyporus sp.MO5多糖诱导白血病细胞株HL-60凋亡作用机制的实验研究 |
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| 引用本文： | 张恒兰,杨炜华,温培娥,任霞,姜国胜,孙晓柏,苏恩裕,姜国胜. 真菌Polyporus sp.MO5多糖诱导白血病细胞株HL-60凋亡作用机制的实验研究[J]. 中华肿瘤防治杂志, 2010, 17(6) |
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| 作者姓名： | 张恒兰 杨炜华 温培娥 任霞 姜国胜 孙晓柏 苏恩裕 姜国胜 |
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| 作者单位： | 山东省医学科学院基础医学研究所,山东省现代医用药物与技术重点实验室,山东省医药卫生肿瘤免疫与中药免疫重点实验室,济南,250062 |
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| 基金项目： | 山东省优秀中青年科学家科研奖励基金(2007BS03002); 国家自然科学基金(30771103); 国家自然科学基金(30600026) |
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| 摘 要： | 目的:研究真菌Polyporus sp.M05多糖对白血病细胞株HL-60增殖抑制及诱导凋亡作用,并探讨其分子机制。方法:活细胞计数法检测多糖的增殖抑制作用;倒置显微镜下直接观察细胞形态;PI染色流式细胞术检测多糖诱导周期阻滞及凋亡作用;RT-PCR检测凋亡相关基因。结果:多糖对HL-60细胞有增殖抑制作用,并随着浓度增大,作用增强;显微镜下观察细胞有明显的凋亡小体。流式细胞术检测细胞有明显G0/G1期阻滞及凋亡峰的出现,并随着时间越长和浓度的增大凋亡阳性率比例越高,与阴性对照相比差异有统计学意义(P<0.01);RT-PCR检测到Bcl-2基因下调,Bax、Fas及Caspase-9基因上调。结论:Polyporus sp.M05中提取的多糖对白血病细胞株HL-60有增殖抑制和诱导凋亡作用,分子机制与Bcl-2基因下调,Bax、Fas及Caspase-9基因上调有关,线粒体途径及死亡受体途径均参与了此凋亡过程。
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| 关 键 词： | 真菌 Polyporus 多糖 HL-60 凋亡 |
Effect and mechanism of apoptosis HL-60 cells induced by a fungus polysaccharide |
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| ZHANG Heng-lan,YANG Wei-hua,WEN Pei-e,REN Xia,JIANG Guo-shen,SUN Xiao-bai,SU En-yu,JIANG Guo-sheng. Effect and mechanism of apoptosis HL-60 cells induced by a fungus polysaccharide[J]. Chinese Journal of Cancer Prevention and Treatment, 2010, 17(6) |
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| Authors: | ZHANG Heng-lan YANG Wei-hua WEN Pei-e REN Xia JIANG Guo-shen SUN Xiao-bai SU En-yu JIANG Guo-sheng |
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| Affiliation: | ZHANG+Heng-lan,YANG+Wei-hua,WEN+Pei-e,REN+Xia,JIANG+Guo-shen,SUN+Xiao-bai,SU+En-yu,JIANG+Guo-shengInstitute+of+Basic+Medicine,Sh,ong+Academy+of+Medical+Sciense,Key+Laboratory+of+Modern+Medicine+,+Technology+of+Sh,ong+Province,Key+Medical+Laboratory+for+Tumor+Immunology+,+Chinese+Medicine+Immunology+of+Sh,ong+Province,Institute+of+Pharmacology+Medicine,Jinan+250062,P.R.China |
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| Abstract: | OBJECTIVE: To study the effect and mechanism of apoptosis of HL-60 cells induced by polysaccharide PSM from Polyporus sp.M05.METHODS: The proliferation and inhibition were studied by the method of leukocyte counting.The cell morphology was detected through a inverted microscope.The cell cycle and apoptosis were analyzed by flow cytometry with propidium iodide staining.Semi-quantitative RT-PCR was used to detect the mRNA expression of the apoptosis gene.RESULTS: PSM significantly inhibited the proliferation ... |
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| Keywords: | fungi Polyporus polysaccharides HL-60 cell apoptosis |
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