二甲基亚硝胺大鼠肝纤维化中医方证研究

目的：探讨二甲基亚硝胺（DMN）大鼠肝纤维化的中医方证。方法：腹腔注射法制备DMN大鼠肝纤维化模型，分别采用5首不同功效的经典方治疗两周，观察各组大鼠肝组织病理学、羟脯氨酸（Hyp）含量及肝功能变化。结果：造模4周后大鼠形成典型小结节性肝硬化，6周后模型组大鼠肝组织损伤较4周时有所缓解。与6周模型组相比，茵陈蒿汤组大鼠肝组织病理变化、Hyp含量及肝功能均显著改善（P〈0．05或P〈0．01）；黄芪汤组大鼠肝组织病理变化、Hyp古量亦显著改善（P〈0．05或P〈0．01）。结论：茵陈蒿汤、黄芪汤能够有效逆转DMN大鼠肝纤维化，成型期DMN肝纤维化大鼠的主要中医方证为湿热、瘀热内蕴兼气虚。

Study on TCM Recipe and Syndrome of Dimethylnitrosamine-induced Hepatic Fibrosis in Rats

Objective:To probe into TCM recipe and syndrome of dimethylnitrosamine(DMN)-induced hepatic fibrosis of the rat.Methods:DMN rat hapetic fibrosis model was prepared by intraperitoneal injection of DMN,and they were respectively treated with 5 classical recipes with difference functions for 2 weeks.Changes of pathology of the hepatic tissue,hydroxyproline(Hyp) content and hapetic function were investigated.Results:4 weeks after modeling,typical micronodular cirrhosis formed,and 6 weeks after modeling,the hepatic injury in the model group had some remission as compared with that at 4 weeks.Compared with the model group at 6 weeks,pathologic changes of the hepatic tissue,Hyp content and hepatic function improved significantly(P<0.05 or P<0.01) in the Inchenhao Decoction group;pathologic changes of the hepatic tissue and Hyp content in the Huangqi Decoction group improved significantly(P<0.05 or P<0.01).Conclusion:Inchenhao Decoction and Huangqi Decoction can effectively reverse DMN-induced hepatic fibrosis of rats;damp-heat,stagnation of heat in the interior with qi-deficiency are main TCM syndromes of DMN hepatic fibrosis rat at the formative stage.