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| RP-HPLC法测定去甲斑蝥酸钠冻千粉针的含量 | |
| 引用本文： | 潘正,付侃,付晓泰. RP-HPLC法测定去甲斑蝥酸钠冻千粉针的含量[J]. 中国药物应用与监测, 2009, 6(4): 215-217 |
| 作者姓名： | 潘正 付侃 付晓泰 |
| 作者单位： | 1. 重庆邮电大学生物信息学院药物研究所,重庆,400065 2. 新加坡国立大学材料科学与工程系,新加坡,118425 3. 重庆医药工业研究院有限责任公司,重庆,400061 |
| 摘 要： | 目的：建立去甲斑螫酸钠冻干粉针含量测定方法。方法：以Fusion—RP80色谱柱（250mm×4．6mm,4μm）为分离柱,以甲醇．KH2PO4缓冲液（0．1mol·L^-1KH2PO4溶液1000mL,加三乙胺1mL,用磷酸调节pH3．0）（15：85）为流动相,流速为1．0mL·min^-1,检测波长为210nm。结果：去甲斑蝥素浓度在2．0～7．0mg·mL^-1。范围内线性关系良好,r=0．9997,平均回收率为100．19％（n=9）,RSD为0．15％。结论：该方法适用于去甲斑蝥酸钠冻干粉针的质量控制。 |
| 关 键 词： | 反相高效液相色谱法 去甲斑蝥酸钠 含量测定 |
Determination of Sodium Demethylcantharidate for Injection by RP-HPLC |
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| PAN Zheng,FU Kan,FU Xiao-tai. Determination of Sodium Demethylcantharidate for Injection by RP-HPLC[J]. Chinese Journal of Drug Application and Monitoring, 2009, 6(4): 215-217 | |
| Authors: | PAN Zheng FU Kan FU Xiao-tai |
| Affiliation: | 1 College of Bio-lnformation, Chongqing University of Posts and Telecommunications, Chongqing, 400065; 2 Department of Material Engineering and Science, National University of Singapore, Singapore, 118425; 3 Chongqing Pharmaceutical Research Institute Co., Ltd, Chongqing, 400061) |
| Abstract: | Objective： To establish a RP-HPLC method for the determination of sodium demethylcantharidate for injection. Methods： A Fusion - RP80 column （250 mm × 4.6 mm, 4 μm） was used for separation. The mobile phase was methanol-potassium dihydrogen phosphate buffer （adding 1 mL triethylamine to 1 000 mL 0.01 mol · L^-1 potassium dihydrogen phosphate solution and adjusting pH to 3.0 with phosphoric acid）（15 ： 85）. The flow rate was 1.0 mL · min^-1. The detection wavelength was 210 rim. Results： The standard curve was in good linearity over the range of 2.0 - 7.0 mg · mL^-1 （r = 0.999 7） and the average recovery was 100.19% （n = 9） with RSD 0.15%. Conclusion： The method can be used as the quality control of sodium demethylcantharidate for injection. |
| Keywords: | RP-HPLC Sodium demethylcantharidate Content determination |
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