| 大鼠脑的神经干细胞的体外培养、增殖和分化 |
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| 引用本文： | 郑志父,林玲,胡建石,陈贻锴,林建银.大鼠脑的神经干细胞的体外培养、增殖和分化[J].神经解剖学杂志,2003,19(1):33-36. |
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| 作者姓名： | 郑志父 林玲 胡建石 陈贻锴 林建银 |
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| 作者单位： | 福建医科大学分子医学研究中心,福州,350004 |
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| 基金项目： | 福建省科技计划重大项目(2001Z020)资助 |
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| 摘 要： | 采用无血清培养基分离和培养大鼠脑的神经干细胞,并通过免疫荧光细胞化学技术进行细胞自我更新能力、巢蛋白表达和多向分化潜能的鉴定。鉴定结果表明,所分离培养的细胞为大鼠神经干细胞,具有神经干细胞的特征,并可在体外大量增殖和较长期培养,可经诱导分化为神经元、星形胶质细胞、少突胶质细胞,是进行诱导分化的良好细胞模型。本文讨论了神经干细胞传代培养中应予以注意的问题和对策。
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| 关 键 词： | 神经干细胞 培养 增殖 分化 大鼠 |
| 修稿时间： | 2002年7月8日 |
CULTURE, PROLIFERATION AND DIFFERENTIATION OF NEURAL STEM CELLS FROM RAT BRAIN IN VITRO |
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| Zheng Zhihong,Lin Ling,Hu Jianshi,Chen Yikai,Lin Jianyin.CULTURE, PROLIFERATION AND DIFFERENTIATION OF NEURAL STEM CELLS FROM RAT BRAIN IN VITRO[J].Chinese Journal of Neuroanatomy,2003,19(1):33-36. |
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| Authors: | Zheng Zhihong Lin Ling Hu Jianshi Chen Yikai Lin Jianyin |
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| Abstract: | Neural stem cells from rat were isolated and cultured using serum-free medium. Their self-renewal ability, expression of Nestin and multipotent of differentiation were identified with immunofluorescence. The cells isolated from rat brain were neural stem cells, which were characterized on their feature, capable of proliferating and maintaining long-term survival in vitro. The cells could be differentiated into neurons, astrocytes and oligodendrocytes. It is a favorable cellular model for studies on inducement of differentiation. Some ambiguous problems were discussed and corresponding opinions were given. |
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| Keywords: | neural stem cell culture proliferation differentiation rat |
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