Adhesion,growth and cytoskeletal characteristics of 8701-BC breast carcinoma cells cultured in the presence of type V collagen |
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| Affiliation: | 2. Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Palermo, Italy;3. Istituto di Istologia ed Embriologia, Università di Palermo, Palermo, Italy;1. Medical University of South Carolina, Charleston, SC, USA;2. University of Pennsylvania, Philadelphia, PA, USA;1. Department of Psychology, Laboratory of Psychopathology and Health Process, University Paris Descartes – Sorbonne Paris Cité, Boulogne-Billancourt, France;2. Division of Medicine, University College London, London, UK;3. Medical School, University of Chile, Santiago, Chile;4. Rheumatology Unit, San Juan de Dios Hospital, Santiago, Chile;1. Porto Alegre Epilepsy Surgery Program, Neurology and Neurosurgery Services, Hospital São Lucas, Brazil;2. The Brain Institute, Brazil;3. School of Medicine, Pontificia Universidade Catolica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil;1. Department of Neurology, Emory University School of Medicine, Atlanta, GA, USA;2. Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA;3. Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, USA;4. Department of Neurology, University of Washington, Seattle, WA, USA;1. Beijing Institute of Functional Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China;2. Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, China;3. Department of Nuclear Medicine, Xuanwu Hospital, Capital Medical University, Beijing, China;4. Department of Radiology, Xuanwu Hospital, Capital Medical University, Beijing, China;5. Department of Pathology, Xuanwu Hospital, Capital Medical University, Beijing, China |
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| Abstract: | ![]()
Type V collagen is one of the minor components of the extracellular matrix (ECM) whose content is increased in cases of ductal infiltrating carcinomas of the breast. In order to clarify its biological role, we have investigated the effect of this molecule, both as substrate and as soluble factor, on the behaviour of a breast carcinoma cell line (8701-BC) grown in vitro. Cell-collagen adhesion was monitored for 24 h from plating in the absence or presence of serum. The influence of type V collagen on cell growth was followed during 9 days of culture, and the actin-vinculin arrangement was studied by simultaneous fluorescent immuno-staining. The results indicate that type V collagen is not a permissive substrate for neoplastic cell proliferation and dissemination in vitro. |
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