VEGF和E-cadherin在胃癌中的表达及意义

目的探讨血管内皮生长因子(VEGF)和钙粘附分子(E-cadherin,E-cad)在胃癌中的表达及意义。方法应用核酸原位杂交方法检测62例胃癌组织中VEGF和E-cad mRNA表达情况。结果62例胃癌中VEGF和E-cadmRNA阳性表达率分别为90.32%(56/62)和32.26%(20/62),VEGF mRNA在胃癌中的阳性表达率显著高于正常胃粘膜上皮组织;E-cad mRNA阳性表达率显著低于正常胃粘膜上皮组织(P<0.01)。VEGF mRNA高表达和E-cadmRNA低表达与胃癌组织分化、TNM分期、淋巴结转移有关(P<0.01)。胃癌中VEGF和E-cad mRNA表达呈负相关(P<0.01)。结论VEGF mRNA过表达与E-cad mRNA失活可加速胃癌的浸润转移。VEGF和E-cad mRNA检测可作为评价胃癌的恶性程度和判断预后的重要指标。

Expression and clinical significances of VEGF and E - cadherin in stomach cancer

Objective To assay vascular endothelial growth factor(VEGF) and E-cadherin(E-cad) expression and outline their clinical significances. Methods A situ hybridization was used to detect the expression of VEGF and E-cad mRNA in 62 patients with stomach cancer. Results The positive rates of VEGF and E-cad mRNA in 62 sample tissues of stomach cancer were 90.32%(56/62) and 32.26% (20/62).The VEGF mRNA staining was more strong in stomach cancer cells than normal gastric mucosa,and the E-cad mRNA staining was weaker(P<0.01).The high expression of VEGF mRNA and the low expression of E-cad mRNA are positively correlated with the differentiation of cancer tissues,the TNM stage and the metastasis of lymph node(P<0.01).In stomach cancer,the expression of VEGF mRNA is adversely associated with E-cad mRNA expression(P<0.01).Conclusion Over expression of VEGF mRNA and inactivation of E-cad mRNA enhance the infiltration and metastasis of stomach cancer.Detecting of VEGF and E-cad mRNA can be served as significant markers for evaluating the malignance and prognosis of stomach cancer.