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| Polymin-HRP核酸杂交探针 | |
| 引用本文： | 孙芝琳,李洪,颜铯,陈俊杰,孙泽芳,林晞. Polymin-HRP核酸杂交探针[J]. 四川大学学报(医学版), 1987, 0(4) |
| 作者姓名： | 孙芝琳 李洪 颜铯 陈俊杰 孙泽芳 林晞 |
| 作者单位： | 华西医科大学生物化学教研室(孙芝琳，李洪，颜铯，陈俊杰，孙泽芳)，华西医科大学生物化学教研室(林晞) |
| 摘 要： | 本文叙述一种将辣根过氧化物酶(HRP)与单链DNA交联以制备非同位素核酸杂交探针的新方法。HRP-单链DNA复合物可用于斑点杂交,经杂文并与HRP底物溶液作用后,可直接检出互补的DNA靶序列,而不需繁冗的细胞化学夹心法。本法可检出 1～4 pg的靶DNA。 |
| 关 键 词： | 核酸分析 杂交 探针 辣根过氧化物酶 |
A POLYMIN-HRP LABELED PROBE FOR DNA HYBRIDIZATION |
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| Sun Zhilin,Li Hong,Yan Se,Chen Junjie,Sun Zefang,Lin Xi. A POLYMIN-HRP LABELED PROBE FOR DNA HYBRIDIZATION[J]. Journal of Sichuan University. Medical science edition, 1987, 0(4) | |
| Authors: | Sun Zhilin Li Hong Yan Se Chen Junjie Sun Zefang Lin Xi |
| Affiliation: | Sun Zhilin;Li Hong;Yan Se;Chen Junjie;Sun Zefang;Lin Xi Department of Biochemistry |
| Abstract: | A new method for constructing nonra-dioactive hybridization probes was studied,which allowed crosslinks to be producedbetween HRP and single-stranded DNA.Such single-stranded DNA-HRP complexeshave been tested for blot hybridization.After hybridization and incubation witha substrate solution, sequences comple-mentary to the probe could be visualizeddirectly without the need of tedious cyto-chemical sandwich methods. This proceduremight detect target DNA in the 1-4 pgrange. |
| Keywords: | Nucleic acids analysis Probe Horseradish peroxidase Hybridization |
| 本文献已被 CNKI 等数据库收录！ | |