苦瓜水提取物对高脂血症小鼠的降血脂作用研究

目的:探讨苦瓜水提取物对高脂血症模型小鼠的降血脂作用.方法:高脂饲养建立高脂血症小鼠模型,随机分成4组,即苦瓜水提取物高、低剂量组(30,10 g·kg-1)、左旋肉碱(1 g·kg-1)组、模型组,另取同步饲养的正常小鼠作为空白对照组,每组10只.连续灌胃给药4周后,眼眶后静脉丛取血,分离血清,检测血清总胆固醇(TC)、甘油三酯(TG)含量；计算肝脏指数,HE染色观察肝脏组织形态学改变.结果:与模型组比较,各给药组小鼠血清TC,TG含量和肝脏指数均明显降低(P ＜0.05,P＜0.01),其中苦瓜水提取物高剂量组小鼠血清TC,TG含量和肝脏指数,与模型组差异非常显著(P＜0.01)；肝组织染色结果显示苦瓜水提取物能明显减少高脂血症小鼠肝脏的脂肪沉积.结论:苦瓜水提取物具有良好的降血脂作用.

Effect of Water Extracts from Momordica charantia on Blood Lipids in Hyperlipidemic Mice

Objective: To investigate the effect of water extracts from Momordica charantia on blood lipid in mouse model of hyperlipidemia. Method: The hyperlipidemia mouse model was induced by high fat diet successfully and the mice were randomly divided into 4 groups:high(30 g·kg^-1) and low(10 g·kg^-1) dose group of water extracts from M. charantia,L-carnitine(1 g·kg^-1)and model group, 10 mice in each group. Another 10 normal mice were used as the control group. After treatment of 4 weeks by gastric gavage, serum total cholesterol (TC)and triglyceride(TG)levels were measured by automatic biochemical analyzer. Liver index was calculated and pathological changes of liver tissue were observed by hematoxylin-eosin(HE) staining. Result: Serum TC, TG and liver index of drug groups were significantly reduced after treatment. TC, TG and liver index in high dose group was (2.78±0.33), (0.90±0.13) mmol·L^-1,(0.034±0.002) g·g^-1;those in low dose group was (3.87±0.08), (1.03±0.09) mmol·L^-1 and (0.040±0.005) g·g^-1. Compared with model group of (4.86±0.34), (1.43±0.06) mmol·L^-1and (0.057±0.007)g·g^-1, there were statistical differences (P<0.01,P<0.05). The HE staining results showed that water extracts from M. charantia could significantly reduce the liver fat content of hyperlipidemic mice. Conclusion: Water extracts from M. charantia can evidently decrease blood lipids.