| 乙型肝炎病毒基因疫苗诱导小鼠产生免疫应答的效应 |
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| 引用本文： | 李文波,张修礼,姚志强. 乙型肝炎病毒基因疫苗诱导小鼠产生免疫应答的效应[J]. 临床消化病杂志, 2003, 15(5): 195-197 |
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| 作者姓名： | 李文波 张修礼 姚志强 |
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| 作者单位： | 济南军区总医院消化科,济南,250031;济南军区总医院消化科,济南,250031;济南军区总医院消化科,济南,250031 |
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| 摘 要： | 目的 :构建编码乙型肝炎病毒 (HBV)表面蛋白S的重组质粒pCR 3 .1 S,将之直接肌肉注射balb/c小鼠 ,观察小鼠HBV特异的免疫应答。方法 :以ELISA法检测小鼠血清 ,3H TdR掺入法测定淋巴细胞增殖 ,51 Cr 4h释放法检测淋巴细胞杀伤功能。结果 :与空载体对照组相比较 ,基因疫苗诱发小鼠产生良好的抗HBs反应及HBV特异的细胞免疫应答 (P <0 .0 5)。结论 :基因疫苗pCR 3 .1 S可诱导balb/c小鼠产生全面的免疫应答
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| 关 键 词： | 乙型肝炎病毒 基因疫苗 免疫应答 |
| 文章编号： | 1005-541X(2003)05-195-03 |
| 修稿时间： | 2003-05-28 |
Gene Vaccines of Hepatitis B Virus Induces Comprehensive Immune Responses in Mice |
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| Li Wen-bo,Zhang Xiu-li,Yao Zhi-qiang. Gene Vaccines of Hepatitis B Virus Induces Comprehensive Immune Responses in Mice[J]. Chinese Journal of Clinical Gastroenterology, 2003, 15(5): 195-197 |
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| Authors: | Li Wen-bo Zhang Xiu-li Yao Zhi-qiang |
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| Affiliation: | Li Wen-bo,Zhang Xiu-li,Yao Zhi-qiang Department of Digestion,General Hospital of Jinan Military Region,Jinan,250031 |
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| Abstract: | Purpose: Recombinant eukaryotic expression plasmids of pCR 3.1-S encoding S protein of hepatitis B virus (HBV) were constructed as gene vaccines.bal b/c mice were inoculated With these plasmids so that immune responses to HBV could be studied. Methods: Antibodies to HBV major proteins were be asured by ELISA titers and specific cellular responses including lymphocyte proliferation and cytotoxic T lymphocyte activity were detected through 3H-TdR incorporation and 4-hour 51Cr release assays.Results:It turns out that mice immunized with gene vaccines have higher anti-HBs and specific cellular responses to HBV than ones with vector plasmids(P<0.05).Conclusion:HBV genetic vaccines successfully induced comprehensive immune responses in bal b/c mice. |
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| Keywords: | Hepatitis B virus Gene vaccines Immune response |
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