瘦素、纤连蛋白对早孕绒毛滋养细胞分泌基质金属蛋白酶的影响

目的探讨瘦素、纤连蛋白对早孕绒毛滋养细胞分泌基质金属蛋白酶的影响及其作用。方法从孕6-8周的人工流产胚胎绒毛中分离、纯化出细胞滋养细胞(CTB)体外培养,在transwell上室CTB培养液中分别加入不同浓度lep-tin,在transwell下室中加入50μg/mlFN,采用明胶酶谱分析法检测培养液中CTB分泌的MMP-2、MMP-9值并进行比较。结果FN组、协同组分泌的MMP-2,9显著高于对照组(P0.05),协同组又高于单加leptin或FN组(P0.05)。结论leptin、FN均具有促进CTB分泌MMPs的作用,且leptin与FN协同作用,更加强了CTB对MMPs的分泌,从而参予调控CTB的浸润能力。

Effects of leptin and fibronectin on the secretion of matrix metalloproteinase in trophoblsts of human first trimester gestation.

Objective：To explore the effects of Leptin and Fibronectin on the secretion of Matrix metalloproteinase in trophoblsts in human first trimester gestation.Methods：Isolated and sublimated Cytotrophoblasts（CTB） from chorionic villi at 6-8 weeks by abortion were cultured in vitro. The CTB cultured in the upper chambers of transwell were added different concentration of leptin,and 50μg/ml FN were added in the lower chamber. MMP-2 and MMP-9 secreted by CTB was detected by Gelatinase spectrum analysis. Results：The purity quotient ANOVA showed significant difference of MMP-2 and MMP-9 levels in groups,P〈0.05.Conclusions：Leptin and FN synergisticly action on CTB could influence the secretion of Matrix metalloproteinases,and so to regulate the invasive ability of CTB.