| 免疫荧光法与 Giemsa 氏法检测沙眼衣原体的比较 |
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| 引用本文： | 周云中,张慈英,李官成.免疫荧光法与 Giemsa 氏法检测沙眼衣原体的比较[J].中南大学学报(医学版),1987(2). |
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| 作者姓名： | 周云中 张慈英 李官成 |
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| 作者单位： | 湖南医学院第一附属医院眼科教研室
(周云中)，湖南医学院第一附属医院中心实验室
(张慈英)，湖南医学院第一附属医院中心实验室(李官成) |
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| 摘 要： | 本文用免疫荧光法与 Giemsa 氏法检测明显沙眼患者,两法阳性率相差无几。但在临床肉眼观察认为正常时,其阳性率分别为24.77%及11.58%(P<0.05),差异有显著意义,表明免疫荧光法检测早期沙眼较 Giemsa 氏法敏感。临床认为正常眼,经实验室检查25.56%为阳性,说明开展沙眼的实验室诊断是必要的。
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| 关 键 词： | 沙眼衣原体 免疫抗体技术 天青染料 对比研究 |
STUDIES OF GIEMSA AND IMMUNOFLUORESCENCE STAINING FOR DETECTION OF CHLAMYDIA TRACHOMATIS |
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| Zhou Yunzhong.STUDIES OF GIEMSA AND IMMUNOFLUORESCENCE STAINING FOR DETECTION OF CHLAMYDIA TRACHOMATIS[J].Journal of Central South University (Medical Sciences)Journal of Central South University (Medical Sciences),1987(2). |
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| Authors: | Zhou Yunzhong |
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| Institution: | Zhou Yunzhong Department of Ophthalmology,The First Affiliated Hospital,Hunan Medical CollegeZhang Ciying,Li Guancheng Central Laboratory,The First Affiliated Hospital,Hunan Medical College |
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| Abstract: | The sensitivities between immunofluorescence staining and Giemsa stainingto detect Chlamydia trachomatis were compared in 209 prominent cases(395eyes)diagnosed clinically.The positive rates in cases with advanced trachomawere 63.89% and 63.64% respectively.In 204 normal eyes examined with bareeyes,the positive rates of the two methods were 24.77% and 11.58%.It showedthat immunofluorescence staining was more sensitive than Giemsa staining indetecting early Stage trachoma(P<0.05).In the 46 patients(90 eyes)while bare-eye method diagnosed as normal 23 eyes(25.56%)revealed positive by thelaboratory method The article suggested that laboratory method could play animportant role in detection of trachoma. |
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| Keywords: | Chlamydia trachomatis Fluorescent antib technic Azune stain Comparative study |
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