麝香保心丸对内皮祖细胞功能及分泌一氧化氮的影响

目的 察麝香保心丸（Shexiang Baoxin Pill）对人外周血内皮祖细胞(endothelial progenitor cells，EPCs)功能及分泌一氧化氮（NO）的影响。

Effects of Shexiang Baoxin Pill on Function and Nitric Oxide Secretion of Endothelial Progenitor Cells

Objective To investigate the effects of Shexiang Baoxin Pill （SBP） on function of endothelial progenitor cells （EPCs） and its nitric oxide （NO） secretion. Methods Total mononuclear cells were isolated from human peripheral blood by ficoll density gradient centrifugation and inoculated on the human fibro-ligandin encrusting plate. After 7 days of in vitro culture, adherent cells were collected and incubated with SBP for 24 h. The proliferation,migration,adhesive activity, vasculogenesis capacity and NO secretion of EPCs were assayed using MTT, Transwell chamber, adhesion determination, in vitro vasculogenesis kit and nitrate reductase method, respectively. Results EPCs incubated with SBP showed the capacities higher than those of control in proliferation, migration, adhesion, in vitro vasculogenesis, and with a higher NO concentration in the culture supernatant. Conclusion SBP can improve the function of EPCs, which might be a novel mechanism of its effects in improving vascular endothelial function and promoting angiogenesis.