| 增龄对成体肌肉干细胞生长能力的影响 |
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| 引用本文： | 邬江,孙晓娟,钟世镇,秦建强,李主一.增龄对成体肌肉干细胞生长能力的影响[J].西南国防医药,2006,16(2):144-146. |
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| 作者姓名： | 邬江 孙晓娟 钟世镇 秦建强 李主一 |
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| 作者单位： | 1. 成都军区昆明总医院全军骨科中心,云南,昆明,650032
2. 南方医科大学临床解剖研究所,广东,广州,510015 |
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| 摘 要： | 目的:探讨不同年龄成体肌肉干细胞—卫星细胞体外培养增殖、分化规律,了解卫星细胞生长能力与年龄的关系。方法:用MTT法、免疫细胞化学技术及粘附细胞仪等手段,检测不同年龄段大鼠多代肌卫星细胞的增殖、分化能力和细胞数量。结果:(1)单位重量细胞数与动物年龄呈负相关;(2)不同年龄组间细胞增殖能力从7月龄始明显递减,分化能力从11月龄始减弱。结论:卫星细胞数量与年龄呈负相关,老年大鼠卫星细胞生长能力显著减弱。
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| 关 键 词： | 干细胞 增殖 分化 |
| 文章编号： | 1004-0188（2006）02-0144-03 |
| 收稿时间： | 2005-07-04 |
| 修稿时间： | 2005年7月4日 |
Effects of aging on growing ability of adult muscle stem cells |
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| WU Jiang,SUN Xiao-juan,ZHONG Shi-zhen,QIN Jian-qiang,LI Zhu-yi.Effects of aging on growing ability of adult muscle stem cells[J].Medical Journal of National Defending forces in Southwest China,2006,16(2):144-146. |
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| Authors: | WU Jiang SUN Xiao-juan ZHONG Shi-zhen QIN Jian-qiang LI Zhu-yi |
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| Abstract: | Objective: To study the rule of proliferation and differentiation of muscle stem cells-satellite cell(SCs) from various age rats in vitro and explore the relationship between SCs and aging.Methods: The quantity and quality of SCs from various age rats were compared by MTT,immune cytochemistry and ACAS.Results: The number of SCs was of a negative correlation with the age.The activity and growing ability of SCs was very stable from 1 month old to 6 months old,but they became worse after 7 months old and the ability of differentiation of SCs reduced rapidly from 11 months old.Conclusion: The number of SCs is related negatively to the age in rats and growing ability of SCs decreased obviously in old rats. |
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| Keywords: | stem cell satellite cell proliferation differentiation |
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