应用改进ELISA检测干血绵球HIV抗体的可行性研究

[目的]研讨以改进的ELISA法检测干血棉球HIV抗体的可行性。[方法]用包括20份S／CO比值在1～3在内的100份HIV抗体阳性血清，198份阴性血清样品进行研究，每份血清分为两部分，一部分血清置-20℃；另一部分血清制成干血棉球置37℃储存3周；然后，用于血棉球与血清样品进行HIV抗体检测对比试验。[结果]（1）血清样品按ELISA说明书方法检测，干血棉球用改进ELISA方法检测，干血棉球与相同的血清样品检测结果之间无显著性差异（P〉0．05）。（2）20份37℃储存3周S／CO比值在1～3的HIV抗体弱阳性干血棉球，用ELISA检测，有部分弱阳性样品出现了假阴性结果，而用改进ELISA法检测，全部阳性。[结论]可用干血棉球代替血清样品，用改进ELISA法进行HIV抗体检测。

Feasibility study on detection of HIV antibody in dry blood cotton with improved ELLSA

[ Objective ] To study the feasibility of improved ELLSA in detection of HIV antibody in dry blood cotton. [ Methods ] 100 HIV antibody positive sera samples, including 20 with the S/CO ratio of 1 - 3, and 198 HIV antibody negative sera samples were used in this study. Each serum was divided into two parts, one was put at -20℃, while the other was made into dry blood cotton put at 37℃ for 3 weeks. Then, the samples of the two parts were conducted comparison test for HIV antibody detection. [ Results] Sera samples was detected in accordance with the ELLSA manual and dry blood cotton was detected by improved ELLSA. It was found there was no difference between the two（P 〉 0.05）. 20 dry blood cotton with S/CO ratio of 1 - 3 put at 37% for 3 weeks and weak positive for HIV antibody, were found some false negative when detected with ELLSA, while all positive when detected with improved ELLSA. [ Conclusion ] Sera samples can be substituted by dry blood cotton with improved ELLSA for HIV antibody detection.