痰瘀同治方对痰瘀互结证冠心病小型猪心肌组织的保护作用

目的：观察痰瘀同治方对痰瘀互结证冠心病小型猪心肌组织的保护作用。方法：将36只中国小型猪随机分为正常对照组、模型组、丹蒌片组和痰瘀同治方高、中、低剂量组（生药2.0，1.0，0.5 g·kg^-1），每组6只；除对照组外，其他各组高脂饲料喂养 2周后，采用介入技术球囊损伤冠状动脉左前降支内皮，术后继续高脂饲料喂养8周，制备小型猪冠心病痰瘀互结证模型；术后给药8周，分别于0周（实验前）、高脂2周（术前或给药前）、高脂6周（给药后4周）、高脂10周（给药后8周），观察各组动物血清超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量；同时实验结束后进行心肌酶谱检测和HE染色病理观察，采用透射电子显微镜观察心肌细胞超微结构的改变。结果：与对照组比较，模型组自第2周起至实验结束，血清SOD活性明显下降，而MDA含量明显上升（P<0.05或P<0.01）；第10周时模型组动物血清肌酸激酶（CK）、乳酸脱氢酶（LDH）和CK-MB水平均明显升高（P<0.05或P<0.01），心肌组织病理形态和超微结构明显异常；与模型组比较，实验结束时痰瘀同治方各剂量组血清SOD活性都有一定程度的上升，而MDA水平则明显下降（P<0.05或P<0.01）；同时痰瘀同治方可明显降低模型动物血清CK和LDH水平（P<0.05或P<0.01），减轻心肌组织缺血性损伤，改善心肌细胞超微结构。结论：痰瘀同治方对痰瘀互结证冠心病中国小型猪心肌损伤有明显保护作用，其机制可能与对抗自由基氧化损伤，抑制脂质过氧化反应相关。

Protective effect of formula of removing both phlegm and blood stasis on myocardial tissues of Chinese mini-swine with coronary heart disease of phlegm-stasis cementation syndrome

Objective: To study the protective effect of formula of removing both phlegm and blood stasis (TYTZ) on myocardial tissues of Chinese mini-swine with coronary heart disease of phlegm-stasis cementation syndrome. Method: Totally 36 Chinese mini-swine were randomly divided to six groups: the normal control group, the model group, the Danlou tablet group, and TYTZ groups with doses of 2.0, 1.0, 0.5 g·kg^-1, with six in each group. Except for the normal control group, all of other groups were fed with high-fat diet for 2 weeks. Interventional balloons are adopted to injure their left anterior descending artery endothelium. After the operation, they were fed with high-fat diet for 8 weeks to prepare the coronary heart disease model of phlegm-stasis cementation syndrome in Chinese mini-swine. After the operation, they were administered with drugs for 8 weeks. The SOD activity and MDA content of each group were observed at the 0^th week (before the experiment), the 2^nd week after the high-fat diet (before the operation or drug administration), the 6^th week after the high-fat diet (4 weeks after the drug administration) and the 10^th week after the high-fat diet (8 weeks after the drug administration). Meanwhile, the myocardial enzymogram test and the HE staining pathological observation were performed at the end of the experiment. The changes in the myocardial cell ultra-structure were observed under transmission electron microscope. Result: Compared with the normal control group, the model group showed significant decrease in serum SOD activity and notable increase in MDA content from the 2^nd week to the end of experiment (P<0.05 and P<0.01). In the 10^th week, the CK, LDH and CK-MB levels in serum also significantly increased in the model group (P<0.05 and P<0.01), with obvious structural abnormality in myocardial tissue pathologic morphology and ultra-structure. Compared with the model group, TYTZ groups showed specific increase in serum SOD activity and oblivious decrease in the MDA level (P<0.05 or P<0.01). Meanwhile, TYTZ could significantly decrease serum CK and LDH levels in the model group (P<0.05 or P<0.01), attenuate the ischemia injury of myocardial tissue, and improve the ultra-structure of cardiomyocytes. Conclusion: TYTZ shows an obvious protective effect on the myocardial injury in Chinese mini-swine with coronary heart disease of phlegm-stasis cementation syndrome. Its mechanism is related to the resistance against free radical oxidation injury and the inhibition of the lipid per-oxidation.