大黄对扑热息痛致急性肝损伤的影响

[目的]探讨扑热息痛致急性肝损伤及中药大黄对该损伤的影响。[方法]70只小鼠随机分为7组,每组10只。通过腹腔注射扑热息痛,造成急性肝损伤动物模型;造模前给予大黄灌胃后分别检测丙氨酸氨基转移酶、天冬氨酸氨基转移酶、γ谷氨酰转肽酶的活性;光镜观察肝脏损伤情况;RT-PCR方法检测肝脏组织肿瘤坏死因子-α（TNF-α）基因表达情况。观察大黄对扑热息痛致肝细胞损伤的影响。[结果]①小鼠一般状态及肝脏组织形态学随扑热息痛作用时间延长逐渐恶化,中药大黄预处理后小鼠一般状态好于同一时间点的扑热息痛组。②与正常对照组比较,扑热息痛和大黄预处理组小鼠血清学指标与TNF-α基因表达量均显著升高（P〈0.01）;与扑热息痛组比较,大黄预处理组小鼠血清指标与TNF-α基因表达量明显下降（P〈0.05）。[结论]大黄能使扑热息痛导致肝细胞损伤小鼠肝脏中TNF-α基因表达上升,从而对肝脏具有保护作用。

Effectiveness of rhubarb in treating paracetamol induced hepatic injury

[Objective]To study the effectiveness of rhubarb in treating paracetamol induced hepatic injury. [Methods]Seventy mice was divided into 7 groups:control group,2 model groups(8th,16th hour after paracetamol injection),4 experimental groups treated by rhubarb(8th,16th hour after paracetamol injection treated by 4 g/kg rhubarb;8th,16th hour after paracetamol injection treated by 8 g/kg rhubarb).The liver injury mice model was induced by paracetamol through intraperitoneal injection.Thereafter,the experimental ...