牙本质磷蛋白对牙本质再矿化的影响

目的　探讨牙本质磷蛋白 (dentinphosphoprotein ,DPP)与脱矿牙本质再矿化的关系。方法　 (1 )用氯化钠提取脱矿牙本质中的易溶性DPP并鉴定。 (2 )用氯化钠去除和未去除易溶性DPP的脱矿人牙根牙本质磨片 ,经再矿化后通过原子吸收光谱、扫描电镜和显微放射照相的观测比较两组再矿化程度。结果　 (1 )证明了 1mol/L氯化钠可以提取脱矿牙本质中的易溶性DPP ;(2 )去除和未去除易溶性DPP的磨片组相比再矿化液的钙离子浓度显著减少 (P <0 0 1 ) ;磨片上生成较多量的矿物质沉积 ;显微射线照片平均吸光度值显著降低 (P <0 0 1 )。结论　易溶性DPP对脱矿牙本质再矿化有明显的抑制作用 ,在根面龋的再矿化中去除易溶性DPP能提高其再矿化的潜能。

Effects of dentine phosphoprotein on remineralization of demineralized dentine

Objective To investigate the relationship between dentine phosphoprotein(DPP) and remineralization of demineralized dentine Methods (1)Soluble DPP was extracted with 1 mol/L NaCl from demineralized dentine and was evaluated (2) Soluble DPP was removed with 0 1 mol/L NaCl or was not removed from demineralized dentine sections in human tooth roots Then all sections were subjected to remineralization treatment, and remineralization degrees were compared by atomic absorption spectrum , SEM and microradiographg Results (1)Soluble DPP was extracted with 1 mol/L NaCl (2)Removal of soluble DPP resulted in significantly lower calcium concentration in remineralization solution( P <0 01),less mean light absorbed value in demineralized dentin sections by microradiography ( P <0 01) Conclusions Soluble DPP may have an inhibiting effect on remineralization of demineralized dentine, this study suggests that the remove of soluble DPP from root caries lesions may enhance their remineralization potential