体外测定CPDA与ACD全血在4℃以上的有效保存期

为确定血液在4℃以上的有效保存期并为战时血液保存与运输提供依据，采集10名健康献血者全血各200ml，每袋分出50ml标记为对照组，其余为实验组。将对照组置于4℃冰箱保存，于保存末期检测红细胞ATP含量为临界ATP；将实验组置于10—33℃下保存，每天测定ATP等指标，以临界ATP为指示点确定有效保存期。结果显示，在10—33℃条件下CPDA全血有效保存期为2．5-18天，ACD全血有效保存期为1—13天。结论：4℃以上保存的CPDA全血可以在有效保存期内运送到前线，使伤员得到快速有效的输血救治。

Measuring the Shelf Life of CPDA and ACD Whole Blood Stored at above 4℃ in vitro

In order to measure the shelf life of whole blood stored at above 4 degrees C and provide experimental data for blood preservation and transportation in battle fields, 200 ml whole blood was collected from each of the 10 donors and anticoagulated by CPDA or ACD, then 50 ml whole blood was separated from each one and marked as control group, the rest was marked as test group. The control group was stored at 4 degrees C and RBC ATP concentrations was measured at the end of its shelf life which signed as critical ATP. The test group was stored at above 4 degrees C condition, some items as ATP, FHb (free hemoglobin), serum K(+) and germiculture were tested daily and ensured all of them eligible. When RBC ATP decreased to the level of critical ATP, the time of preservation was considered as shelf life. The results showed that at temperatures from 10 to 33 degrees C, the shelf life of CPDA whole blood ranges from 2.5 days to 18 days, while shelf life of ACD whole blood ranges from 1 day to 13 days. It is concluded that CPDA whole blood stored at above 4 degrees C condition can be sent to the front hospital in effective shelf life so that the wounded can be cured in time.