| 健脾化痰法对前脂肪细胞分泌功能影响的研究 |
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| 引用本文： | 熊文生,马群英. 健脾化痰法对前脂肪细胞分泌功能影响的研究[J]. 新中医, 2010, 0(4): 84-86 |
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| 作者姓名： | 熊文生 马群英 |
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| 作者单位： | 广州中医药大学第一附属医院二内科; |
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| 基金项目： | 国家自然科学基金课题(编号:30572384) |
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| 摘 要： | 目的:通过检测用含药大鼠血清培养前脂肪细胞分化过程中瘦素、瘦素受体m RNA的表达和脂联素含量,探讨健脾化痰法对脂肪细胞分泌功能的影响。方法:对SD大鼠分别给予健脾化痰方、健脾方、化痰方、维甲酸及生理盐水灌胃7天后,分离血清以培养并诱导前脂肪细胞分化,检测试验指标。结果:健脾化痰法可明显提高脂肪细胞的瘦素分泌,效果优于单纯健脾法或单纯化痰法(P0.05,P0.01),时间因素对瘦素分泌的影响较大。健脾化痰法可明显提高脂肪细胞瘦素受体m RNA的分泌,总体趋势为:健脾化痰组化痰组维甲酸组健脾组空白血清组完全空白组。健脾化痰法可明显提高脂肪细胞脂联素的含量,在所有时间段效果均优于其他各组(P0.05,P0.01)。结论:健脾化痰法可明显提高脂肪细胞的瘦素、瘦素受体m RNA和脂联素的分泌,效果优于单纯健脾法或单纯化痰法。
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| 关 键 词： | 前脂肪细胞 健脾化痰法 瘦素 脂联素 动物实验 大鼠 |
Effect of Therapy of Strengthening Spleen and Resolving Phlegm on Secretary Function of Pre-adipocytes |
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| XIONG Wensheng,MA Qunying. Effect of Therapy of Strengthening Spleen and Resolving Phlegm on Secretary Function of Pre-adipocytes[J]. JOURNAL OF NEW CHINESE MEDICINE, 2010, 0(4): 84-86 |
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| Authors: | XIONG Wensheng MA Qunying |
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| Abstract: | Objective:To observe the effect of therapy of strengthening spleen and resolving phlegm on the levels of leptin,leptin receptor mRNA expression and adiponectin secreted by pre-adipocytes cultured with rats drug-containing serum.Methods:Drug-containing serum was prepared by feeding the rats with ratinoic acid,normal saline,and herbs with actions of strengthening spleen and resolving phlegm,strengthening spleen or resolving phlegm for 7 days.Then the serum was isolated and used for culturing and inducing diff... |
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