羊膜自噬的形态及其相关基因表达

目的：阐明羊膜上皮细胞自噬过程的形态及其相关基因表达。方法：20片足月羊膜用透射电镜检查，用单丹磺酰戊二胺（MDC）标记自噬泡和溶酶示棕红（LTR）检查溶酶体；用RT—PCR检测beclin-1，apg-5和DAPK基因的转录。结果：在羊膜上皮可见多数双层膜包被的自噬泡，它可分为两种：一种是自噬体，其中包含细胞浆成份；另一种是自噬溶酶体。系自噬体和溶酶体融合。细胞浆成份降解。MDC和LTR染色阳性。beclin-1，apg-5和DAPK基因在多数羊膜上皮有表达。结论：在正常羊膜上皮出现自噬并有相关基因表达，羊膜上皮为适应其营养限制可诱导自噬以促进自我营养。

Morphology of autophagy and expression of some relative genes in amnion

Objective: To expose the morphology of autophagy and expression of some associated genes in the amnion. Methods: Twenty pieces of amnions in term gestation were examined by transmitted electron microscopy and by mono-dansylcadaverin (MDC) and LysoTracker Red (LTR) staining, markers for autophagic vacuole and lysosome. Autophagy-as-sociated genes, beclin-1, apg-5 and DAPK were assayed by RT-PCR. Results:Most autophagic vacuoles enclosed with double membranes were found in the amniotic epithelial cells. They were divided two kinds: autophagosomes, in which contained some cytoplasmic compartments, and autolysosomes, in which autophagosome fused with lysosomes and contained degraded cytoplasmic compartments. MDC and LTR staining was positive in the cytoplasm. beclin-1, apg-5 and DAPK genes were expressed in great proportion of amniotic epithelium. Conclusion:These results display that the phenotype of autophagy are induced in normal amniotic epithelium with some autophagy-associated genes expression, and suggest that amniotic epithelia adapt to the nutrient limitation by inducing autophagy to promote self-nourishment.