大黄灵仙胶囊对胆结石防治作用机制的实验研究

目的 运用现代医学实验方法,从生物化学、分子生物学水平研究和探讨大黄灵仙胶囊对胆结石的防治作用机制.方法 选健康新西兰大白兔共80只,分为模型组、大黄灵仙组、消炎利胆组和正常对照组4组,每组20只.模型组、大黄灵仙组、消炎利胆组进行慢性肝损伤造模,完成后大黄灵仙组和消炎利胆组分别予大黄灵仙胶囊（制作成悬液）、消炎利胆片（制作成悬液）灌胃;模型组和对照组以等量生理盐水灌胃.6周后取材,观察、比较胆囊成石情况;检测各组兔血清相关指标;检测兔肝组织总RNA和肝组织中CYP7A1mRNA和acbc11mRNA基因表达相对量.结果 大黄灵仙组在降低胆固醇含量、增加胆汁酸含量方面结果 优于消炎利胆组;模型组兔肝组织中CYP7A1和acbc11基因表达显著下调,分别只有正常组的54.0%和49.9%;大黄灵仙组、消炎利胆组兔肝组织CYP7A1mRNA和胆汁酸盐输出泵acbc11mRNA表达量亦比正常组下降,但却比模型组有明显上升趋势,同时比较二者治疗结果,大黄灵仙组优于消炎利胆组（P〈0.05）,特别是在改善acbc11基因表达方面更具优势.结论 大黄灵仙胶囊能够修复受损肝细胞,稳定肝细胞CYP7A1、胆汁酸盐输出泵acbc11的基因表达,改善肝脏胆固醇代谢和胆汁酸代谢等,有效地保护了肝脏功能,起到了减少和预防结石形成的作用.

Experimental study of the effect of dahuanglingxian capsule in the prevention and treatment of gallstones

Objective By modern medical experimental methods, at the level of biochemistry and molecular biology,to explore the mechanism of dahuanglingxian capsule in prevention and treatment of gallstones. Methods Eighty health New Zealand White rabbits were selected and randomly divided into 4 groups： model groups, dahuan- glingxian group, xiaoyanlidan group and normal control group 4 groups, with 20 rabbits in each group. For the model group, dahuanglingxian group and xiaoyanlidan group, they were made into the model of chronic liver injury. The dahuanglingxian group received dahuanglingxian capsules by garage which were made into suspension, and the xiaoy- anlidan group received xiaoyanlidan tablets by garage which were made into suspension, while the control group were given saline water intragastrically. After 6 weeks observation, the samples were collected and the conditions of gall- bladder stones were observed and compared. The related indexes in serum of rabbit were detected; the total RNA were detected in rabbit liver tissue, the gene expression relative amount were detected. Results The dabuanglingxian group was better than the xiaoyanlidan group in decrease of cholesterol conent and increase of bile acid content. CYP7A1 and acbcll gene expression in liver tissue in the＇model group were down-regulated significantly and were 54% and 49. 9% of the normal control group＇ s; in dahuanglingxian group and xiaoyanlidan group CYP7A1mRNA and aebel 1 mRNA amount in rabbit liver tissue were lower than normal group, but were higher than model group, having a clear ascendant trend. At the same time, There were significant differences in therapeutic effect between the two groups. Effect of dahuanglingxian groups was better than that of xiaoyanlidan group（P 〈 0. 05） ,particularly in impro- ving the expression of acbcl 1 gene. Conehmion Dahuanglingxian capsules can repair damaged liver cells, stabilize the gene expression of CYPTA1 in liver cells and bile salt export pmps acbcl 1,improve the metabolism of cholesterol and bile acid in the liver, effectively protect the liver function, and play a role in the reduction and prevention of gallstone formation.