HPLC法同时测定复方槐花口服液中柚皮苷和黄芩苷的含量

目的:建立HPLC法同时测定复方槐花口服液中柚皮苷、黄芩苷的含量.方法:色谱柱:Agilent ZORBAX SB- C18(250 mm×4.6 mm,5 μm);流动相:乙腈-0.1%磷酸溶液(23:77);检测波长:280 nm;流速:1.0 ml·min-1;柱温:30℃.结果:柚皮苷和黄芩苷分别在0.194～1.742 μg·ml-1(r=0.999 5,n=5)和0.419～3.773 μg·ml-1(r=0.999 8,n=5)范围内与峰面积呈良好线性关系,平均加样回收率为100.06%,RSD为1.10%(n=6)和99.27%,RSD为1.43%(n=6).结论:HPLC法操作简便,结果准确,可用于复方槐花口服液的质量控制.

Determination of Baicalin and Naringin in Compound Huaihua Oral Liquids by HPLC

Objective： To develop an HPLC method for the determination of naringin and baicalin in compound Huaihua oral liquids. Method： The determination was carried out on an Agilent ZORBAX SB- Cls （250 mm × 4.6 mm,5 μm） column with acetonitrile- 0.1% phosphoric acid （23： 77） as the mobile phase. The flow rate was 1.0 ml min-1 and the UV detection wavelength was set at 280 nm with the column temperature of 30℃. Result： The linearity of naringin and baicalin was within the range of 0. 194-1. 742μg m- 1 （ r = 0. 999 5, n = 5 ） and 0.419-3. 773 μg ml -1 （ r = 0. 999 8, n = 5 ）, respectively. The average recovery of baicalin and naringin was 100.06% and 99.27% with RSD of 1.10% （ n = 6） and 1.43% （ n = 6）, respectively. Conclusion： The method is simple, accurate and specific, and can be used in the quality control of compound Huaihua oral liquids.