RP-HPLC法测定人血清中地高辛浓度

目的建立测定人血清中地高辛浓度测定的方法。方法将血清酸化,用丙醇∶氯仿（1∶5）萃取2次,吹干萃取液,残渣用流动相溶解,采用高效液相色谱法测定,色谱柱：Hypersil ODS C18柱（250 mm×4.0 mm,5μm）,流动相：甲醇∶水（60∶40）,检测波长：220 nm。结果地高辛浓度在0.35~2.35 ng/mL范围内,地高辛和内标的峰面积比与浓度呈现良好线性关系（r=0.995 8）,平均回收率103.2%~106.4%,日内和日间精密度RSD〈7.5%（n=5）。结论该方法快速、简便、准确,适合于临床血药浓度监测。

Determination of Digoxin in Serum by RP-HPLC

Objective To establish a method for the determination of Digoxin in serum.Methods The samples were acidified at first,then extracted twice by propyl alcohol-chloroform（5∶1）,and the extraction were blew to dry,the leavings were dissolved by the mobile phase at last.With determination by RP-HPLC,analytical column was Hypersil ODS C18（250×4.0 mm,5 μm）,the mobile phase was consisted of methanol-water（60∶40）,detected at wavelength 220 nm.Results The linear rang of Digoxin was 0.35～2.35ng/mL.The mean relative recovery arranged from 103.2% to 106.4%.The relative standard deviation of within day and between day were all less than 7.5%（n=5）.Conclusion This method is found to be rapid,accurate,sensitive and more suitable for clinical TDM.