| 三氧化二砷抑制NCI-H69肺癌细胞增殖的初步研究 |
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| 引用本文： | 姚和瑞,谢德荣,向燕群,陈岱佳,曾宪平. 三氧化二砷抑制NCI-H69肺癌细胞增殖的初步研究[J]. 中国癌症杂志, 2000, 10(5): 428-429,437 |
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| 作者姓名： | 姚和瑞 谢德荣 向燕群 陈岱佳 曾宪平 |
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| 作者单位： | 1. 中山医科大学孙逸仙纪念医院肿瘤科,广东广州,510120
2. 中山医科大学孙逸仙纪念医院内科,广东广州,510120 |
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| 摘 要： | 目的：观察三氧化二砷（ＡＳ２Ｏ３）对肺癌细胞株ＮＣＩ－Ｈ６９的增殖抑制作用并初步探讨其机制。方法：肺癌细胞株ＮＣＩ－Ｈ６９用不同浓度ＡＳ２Ｏ３处理。ＭＴＴ法观察不同浓度ＡＳ２Ｏ３作用不同时间后对ＮＣＩ－Ｈ６９生长的影响；增殖细胞核抗原（ＰＣＮＡ）单抗ＬＳＡＢ免疫组化染色－流式细胞仪检测ＰＣＮＡ阳性细胞百分比。结果：ＡＳ２Ｏ３明显抑制肺癌细胞株ＮＣＩ－Ｈ６９细胞增殖，其作用随ＡＳ２Ｏ３浓度增加和作用
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| 关 键 词： | 肺肿瘤 三氧化二砷 PCNA 细胞增殖 |
| 文章编号： | 1007-3639(2000)05-0428-02 |
Proliferation inhibition induced by arsenic troxide on lung neoplasms cell line NCI-H69 |
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| YAO He rui,XIE De rong,XIANG Yan qun,et al. Proliferation inhibition induced by arsenic troxide on lung neoplasms cell line NCI-H69[J]. China Oncology, 2000, 10(5): 428-429,437 |
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| Authors: | YAO He rui XIE De rong XIANG Yan qun et al |
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| Abstract: | Purpose:To observe the effect of proliferation inhibition of Arsenic Trioxide(AS 2O 3) on lung cancer cell line NCI H69.Methods:Lung cancer cell line NCI H69 was treated with different concentrations of AS 2O 3. MTT assay was used to observe the proliferation status. Flow cytometry was used to measure the percentage of proliferating cell nuclear antigen (PCNA) positive cell. Results:AS 2O 3 could significantly inhibit the growth of NCI H69 cell, this effect was dose and time dependent. The percentage of PCNA positive cell was decreased gradually when concentration of AS 2O 3 was increased. Conclusions:Arsenic Trioxide could inhibit the proliferation activity of lung cancer cell line SW620. Reduction of PCNA which was related to the regulation of DNA synthesis and cell proliferation may be the mechanism of this proliferation inhibition. |
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| Keywords: | lung neoplasms arsenic trioxide proliferating cell nuclear antigen |
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