重叠感染SEN病毒对乙肝后肝硬化发展的影响

目的：通过检测慢性乙型肝炎和乙肝肝硬化患者SEN病毒（SENV）的感染情况，了解重叠感染SENV对乙肝后肝硬化发展的影响。方法：采用巢式聚合酶链反应（nPCR）对148例慢性乙型肝炎患者、119例乙肝肝硬化患者和80例健康体检者进行SENV—D／H亚型的检测，并对部分阳性血清的PCR产物进行测序。结果：SENV—H在乙肝肝硬化患者血清中的检出率（为37．8％）高于慢性乙型肝炎患者（为26．4％），两者差异有显著性（P〈0．05），而SENV—D的检出率分别为34．5％和30．4％，两者差异无显著性（P〉0.05）；健康体检者血清中，SENV—H和D的检出率分别为13．8％和16．3％，与上述两组患者相比差异有显著性（P〈0.05）；此外，在77例有输血史患者和190例无输血史患者血清中，SENV的总检出率分别为53．2％和55．8％，两者差异无显著性（P〉0.05）。结论：重叠SENV—H感染可能对乙肝后肝硬化发展具有一定的影响，并且SENV的主要传播方式除输血外可能还存在其他途径。

Effect of SEN virus infection on the incidence of liver cirrhosis in patients with chronic hepatitis B

Objective: To investigate the influence of SEN virus infection on the incidence of liver cirrhosis in patients with chronic hepatitis B (CHB). Methods: SENV-D and SENV-H DNA were detected in 148 cirrhosis patients with CHB,119 CHB patients and 80 healthy blood donors with nested-PCR. Some PCR products were sequenced. Results: By sequencing,the specificity of genotypespecific PCR was confirmed. The positive rates of SENV-H were obviously higher in cirrhosis patients with CHB than that in CHB patients(37.8% vs 26.4%,P <0.05). The prevalence of SENV-D/H viremia was signifi-cantly higher in patients with liver cirrhosis and CHB than that in healthy blood donors (SENV-D: 34.5%, 30.4% vs 16.3%;SENV-H: 37.8%,26.4% vs 13.8%;P <0.05). No significant difference was found for SENV-H between patients with transfusion and ones without transfusion (53.2% vs 55.8%,P>0.05). Conclusion: Coinfection with SENV-H may influence the incidence of liver cirrhosis in patients with chronic hepatitis B,and transfusion is not single route of transmission of SEN virus.