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酶联免疫法筛查人类免疫缺陷病毒抗体在艾滋病诊断中的应用
引用本文:冯燕兰. 酶联免疫法筛查人类免疫缺陷病毒抗体在艾滋病诊断中的应用[J]. 医疗装备, 2021, 0(1): 44-45
作者姓名:冯燕兰
作者单位:茂名市妇幼保健院临检室
摘    要:
目的 探讨酶联免疫法筛查人类免疫缺陷病毒(HIV)抗体在艾滋病诊断中的应用效果.方法 选择2019年1—12月于医院接受HIV抗体筛查的1000例艾滋病疑似患者作为研究对象,所有患者均行酶联免疫法与金标斑点法检测,以初筛及印证试验联合诊断结果 为金标准,比较酶联免疫法与金标斑点法的诊断准确度、灵敏度、特异度.结果 酶联...

关 键 词:酶联免疫法  艾滋病  人类免疫缺陷病毒抗体  诊断价值

The Application of Screening Human Immunodeficiency Virus Antibody by Enzymelinked Immunosorbent Assay in Diagnosing AIDS
Feng Yanlan. The Application of Screening Human Immunodeficiency Virus Antibody by Enzymelinked Immunosorbent Assay in Diagnosing AIDS[J]. Medical Equipment, 2021, 0(1): 44-45
Authors:Feng Yanlan
Affiliation:(Clinical Laboratory,Maoming Martenal and Child Health Hospital,Maoming Guangdong 525000,China)
Abstract:
Objective The application effect of screening human immunodeficiency virus(HIV)antibody by enzyme-linked immunosorbent assay(ELISA)in diagnosing AIDS was explored.Methods A total of 1000 suspected AIDS patients who have received HIV antibody screening in the hospital from January to December in 2019 were selected as the subjects in this study.All patients were tested by(ELISA)and dot immunogold assay.The results of preliminary screening combined with verified tests were defined as the gold standards and then the diagnostic accuracy,sensitivity and specificity were compared between ELISA and dot immunogold assay.Results The diagnostic accuracy of ELISA(99.90%)was higher than that of dot immunogold assay(98.90%),and the difference was statically significant(P<0.05).The diagnostic sensitivity and specificity of ELISA(100.00%,99.80%)were both higher than those of dot immunogold assay(75.00%,99.00%),and the differences were statistically significant(P<0.05).Conclusion The application value of ELISA in screening HIV antibody for diagnosing AIDS was relatively high,thus could providing effective references for clinical diagnosing.
Keywords:Enzyme-linked immunosorbent assay  AIDS  Human immunodeficiency virus antibody  Diagnostic value
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