酪蛋白磷酸肽-磷酸钙溶液抑制牛牙早期釉质龋的体外研究

目的 研究酪蛋白磷酸肽-磷酸钙溶液(CPP-ACP)在体外对牛牙釉质早期人工龋的抑制作用.方法 将28颗牛牙釉质样本随机分成4组,每组7颗,浸入唾液24 h以形成人工获得性膜,然后用4种不同溶液(A组:去离子水;B组:1% CPP-ACP溶液;C组:0.05%的NaF溶液;D组:1% CPP-ACP和0.05% NaF...

Study on Effect of CPP-ACP in Inhibiting Bovine Enamel Demineralization in vitro

Objective To investigate the effect of casein phosphopeptide-stabilized amorphous calcium phosphate solution(CPP-ACP) in inhibiting bovine enamel demineralization in vitro.Methods Twenty-eight enamel pieces were randomly divided into four groups(n=7),and immersed in saliva for 24 hours to obtain acquired pellicle.Each group was given different treatments(Group A: deionized water;Group B: 1% CPP-ACP;Group C: 0.05% NaF;Group D: 1% CPP-ACP+0.05% NaF) for 7 days,and immersed in fresh streptococcus mutans solution for 7 days to produce artificial caries.Finally,all samples were detected by the scanning electron microscope(SEM) and the surface microhardness(SMH) machine.Results The SEM showed lots of sizes concane on the enamel surface of group A.While the enamel surface of the other three groups were completed,and a large number of deposits were found on the surfaces.The SMH of groups B,C and D were higher than group A(P<0.05).In groups B,C and D,there were significant differences between each other(P<0.05).Conclusion CPP-ACP can reduce enamel demineralization and promoting remineralization in vitro.CPP-ACP and NaF have synergistic effect in caries prevention.