结、直肠癌花生凝集素受体的研究

应用自己制备的花生凝集素(PNA)单克隆抗体和ABC免疫组织化学方法结合其他三种凝集素(蓖麻凝集素、伴刀豆凝集素和荆豆凝集素)检测PNA对270例各种肿瘤和正常结、直肠粘膜结合的情况。30例结肠癌、71例直肠癌、14例胃癌、12例食道癌和87例各种非消化道的恶性肿瘤,PNA阳性率分别为76.6％,50.7％,7.1％,16.6％和1.1％。30例正常结、直肠粘膜及26例各种良性病变PNA标记均为阴性。PNA标记的阳性率,对消化道癌肿显著高于非消化道的癌肿,消化道癌肿中结、直肠癌显著高于胃与食道癌,肠癌中结肠癌显著高于直肠癌,结果提示PNA是一种对结、直肠癌的诊断与鉴别诊断,新的有用工具。

A STUDY ON THE PEANUT AGGLUTININ RECEPTOR IN COLORECTAL ACARCINOMA

PNA binding was shown by the ABC immunohistochemica method using PNA monoclonal antibody (prepared in our laboratory), 270. cases of variegated tumors and normal colorectal mucosa were screened. The positive rate in 30 cases of colon oarcinoma was 76.6A%; in 71 cases of rectal carcinoma, 50.7%; in 14 cases of stomach oaroinoma, 7.1%; in 12 oases of esophageal carcinoma; 16.6% and in 87 cases of nonalimentary tract malignant tumors, 1.1%, In 30 cases of normal oolorectal mucosa and 26 cases of various benign lesions, PNA binding was all negative. More positive oases were found in alimentary tract tumors than in non-alimentary tract tumors (x2=53,04, p<0.01); more positive oases were found in colorectal carcinoma than in gastric and esophageal carcinomas (x2 = 18.18, p< 0.01); more positive oases were found in colon carcinoma than in rectal carcinoma (x2=5.84, p<0.025). These results suggest that PNA might be, used as a new probe for the diagnosis and differential diagnosis of oolorectal carcinoma.