| 巯基化羧甲基壳聚糖微凝胶的制备及其生物黏附性能*☆ |
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| 引用本文： | 徐咏梅,周伟,殷 磊,王 乐,江 澜,沈 元. 巯基化羧甲基壳聚糖微凝胶的制备及其生物黏附性能*☆[J]. 中国神经再生研究, 2009, 13(29): 5645-5648 |
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| 作者姓名： | 徐咏梅 周伟 殷 磊 王 乐 江 澜 沈 元 |
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| 作者单位： | 武汉理工大学化工学院，湖北省武汉市 430070,武汉理工大学,武汉理工大学化工学院，湖北省武汉市 430070,武汉理工大学化工学院，湖北省武汉市 430070,武汉理工大学化工学院，湖北省武汉市 430070,武汉理工大学化工学院，湖北省武汉市 430070 |
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| 基金项目： | 国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 摘 要： | 摘要：巯基化羧甲基壳聚糖是羧甲基壳聚糖的衍生物。它既保留了羧甲基壳聚糖的优良生物特性，具备水溶性和pH敏感性，同时也具备了优良的生物黏附性，是制备生物凝胶材料的优良原料。利用L-半胱氨酸进行巯基反应制备巯基化羧甲基壳聚糖，通过1-乙基-3- (3-二甲胺基丙基)-碳二亚胺盐酸盐交联和自体交联等方式制备了微凝胶，并以猪胃黏蛋白为模型，测定微凝胶的体外生物黏附性。实验表明巯基化羧甲基壳聚糖粉末在pH3.0的介质中有最大黏附率(120.25%)，表现出了优良的生物黏附性能，在相同的介质中，自身交联的巯基化羧甲基壳聚糖微凝胶的黏附能力要大于1-乙基-3- (3-二甲胺基丙基) -碳二亚胺盐酸盐交联的微凝胶。
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| 关 键 词： | 巯基化羧甲基壳聚糖；微凝胶；生物粘附 |
| 修稿时间： | 2009-06-05 |
Preparation and bioadhesion of micro-gel of thiolated carboxymethyl chitosan |
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| Xu Yong-mei,Zhou Wei,Yin Lei,Wang Le,Jiang Lan and Shen Yuan. Preparation and bioadhesion of micro-gel of thiolated carboxymethyl chitosan[J]. Neural Regeneration Research, 2009, 13(29): 5645-5648 |
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| Authors: | Xu Yong-mei Zhou Wei Yin Lei Wang Le Jiang Lan Shen Yuan |
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| Affiliation: | College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, Hubei Province, China,College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, Hubei Province, China,College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, Hubei Province, China,College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, Hubei Province, China,College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, Hubei Province, China,College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, Hubei Province, China |
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| Abstract: | Abstract: Thiolated carboxymethyl chitosan (SH-CMC), the derivative of carboxymethyl chitosan (CMC), is a water-soluble and pH sensitive material, while retained the excellent bioadhesion, so it is a superexcellent material to prepare biogelatin. L-cysteine was used to prepare the SH-CMC. Micro-gel based on SH-carboxymethyl chitosan was prepared by crosslinking with 1-ethyl-3-(3-dimethylamino-propyl)-carbodiimide hydrochloride (EDC) and itself. The in vitro biological adhesion of micro-gel was determined, and pig gastric mucin was used as a model protein. Experiments showed that SH-carboxymethyl chitosan the biological adhesion of micro-gel had the biggest rate of 120.25% in pH3.0. In the same medium, the adhesion ability of micro-SH CMC gel crosslinked by itself is greater than EDC crosslinking. |
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| Keywords: | Carboxymethyl Chitosan/Chitin Micro-gel Biological adhesion |
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