胆管癌组织及胆汁中端粒酶活性的检测及意义

目的：检测胆管癌组织和胆汁中的端粒酶活性，以研究与端粒酶与胆管癌的关系及对胆管癌的诊断意义。方法：用PCR—ELISA方法检测20例胆管癌的癌组织和胆汁中端粒酶活性，同时用相同的方法检测20例正常胆管组织和胆汁中的端粒酶活性以作对照。结果：20例胆管癌组织中，端粒酶活性阳性为80％（16／20），胆汁中端粒酶活性阳性率75％（15／20）。正常胆管组织端粒酶活性阳性表达率为0（0／20），胆汁中端粒酶活性阳性率为0（0／20）。结论：端粒酶可能参与了胆管癌的发生发展过程，检测胆汁中端粒酶活性可有助于胆管癌的诊断。

Determination and significance of telomerase activity in bile duct carcinomas and the bile

Objective:To detect the telomerase activity in bile duct carcinomas and their bile and to elucidate the relationship between telomerase and bile duct carcinoma and its significance in clinical diagnosis.Methods:Telomerase activities were examind in 20 cases of bile duct carcinoma and their bile,and 20 cases of normal bile duct tissues and their bile respectively with PCR-ELISA method.Results:Telomerase activity was detected in 16 of 20 cases(80%) of bile duct carcinoma,telomerase activity was detected in 15 of 20 cases(75%) of bile of bile duct carcinoma,and was not found in 20 cases of normal bile duct tissue and their bile.Conclusion:The telomerase is most likely involved in the proliferation and malignant transformation of bile epithelia and the malignant progression of bile duct carcinoma,the detection of telomerase activity in bile may be useful for diagnosis of bile duct carcinoma.