耐亚胺培南绿脓假单胞菌的体外耐药监测与基因分型研究

目的 探讨对亚胺培南耐药的绿脓假单胞菌（IRPA）的耐约特征及流行现状，以利于医院感染的监测控制。方法 采用肠杆菌科基因间重复一致序列PCR（ERIC-PCR）方法对56株重症监护病房（ICU）分离的IRPA进行分子生物学分型；采用琼脂稀释法检测8种抗菌药物对IRPA的最低抑菌浓度（MIC）。结果 56株IRPA用ERIC方法分为33刊；IRPA对5种抗菌药物的耐药率≥50．0％。结论 IRPA的多重耐药性值得关注，ICU内IRPA存在散在的克隆流行态势，应注意监控IRPA在医院暴发流行。

Antibiotics resistance profile and ERIC-PCR fingerprinting of Pseudomonas aeruginosa isolates resisting to imipenem

Objective To explore antibiotics resistance profiles and DNA fingerprints of Pseudomonas aeruginosa isolates resistant to imipenem (IRPA). Methods DNA fingerprints of 56 strains isolated from ICU (intensive care unit) were constructed by ERIC-PCR (enterobacter repetitive intergenic consensus-PCR). MICs (minimal inhibitory concentrations) were determined by agar dilution method.Results 33 genotypes were got from 56 strains by ERIC-PCR. Of 8 frequently used antibiotics, 5 of them showed resistance rate higher than 50%. Conclusion It is high time to pay attention to multi-drug resistance of IRPA. The exist of prevalence of IRPA clone in ICU advise us to control of IRPA in hospital effectively.