新生大鼠海马神经干细胞表达趋化因子受体CX3CR1的体外研究

目的 探讨体外培养神经干细胞是否表达趋化因子受体CX3CR1.方法 采用无血清方法分离、培养新生大鼠海马神经干细胞,细胞免疫荧光方法检测神经干细胞标志巢蛋白(nestin)表达及干细胞多向分化为神经元及胶质细胞的能力,后通过细胞免疫荧光及RT-PCR方法检测神经干细胞表达趋化因子受体CX3CR1的情况.结果 体外培养新生大鼠海马神经干细胞呈nestin阳性.可分化为神经丝蛋白200(NF200)阳性的神经元、胶质纤维酸性蛋白(GFAP)阳性的星形胶质细胞及2',3'-环核苷酸-3'-磷酸二酯酶(CNP)阳性的少突胶质细胞,细胞免疫荧光及RT-PCR证实神经干细胞表达趋化因子受体CX3CR1.结论 新生大鼠海马神经干细胞表达趋化因子受体CX3CR1.为进一步研究其体内外迁移提供理论依据.

In vitro study on expression of chemokine receptor CX3CR1 in neural stem cells of neonatal rat hippocampi

Objective To explore whether neural stern cells in vitro express the chemokine receptor CX3CR1. Methods The neural stem cells were isolated from the hippocampi of neonatal rats and cultured in a serum-flee medium. Using immunofluorescence, the neural stem cells and their differentiation into neural and glial cell types were identified; then, the immunofluorescence and RT-PCR were employed to detect whether the neural stem cells in vitro expressed chemokine receptor CX3CR1. Results The floating neural spheres, isolated from neonatal rat hippocampi, were nestin positive and could differentiate into cells to express characteristic antigens of neurons, astrocytes and oligodendrocytes, Immunofluorescence and RT-PCR showed that neural stem cells expressed chemokine receptor CX3CR1. Conclusion Neural stem cells in vitro can express chemokine receptor CX3CR1, which offers the theoretical evidence for further study of its migration.