激活HaCat细胞表面TLR促进细胞迁移的初步研究

目的观察长链游离脂肪酸对体外培养的角质形成细胞的作用,探讨其促进细胞迁移的机制。方法 (1)体外培养HaCat细胞。(2)免疫印迹法(Weston bloten)检测HaCat细胞表面TLR2、TLR4表达。(3)划痕实验法检测激活TLR对HaCat细胞迁移率的影响。(4)Real Time-PCR法分析N、PA、SA、LPS组MMP-3、MMP-9、TIMP-1的表达。结果 (1)HaCat细胞表面有丰富的TLR2、TLR4受体表达。(2)SA、LPS激活细胞表面TLR后可以明显促进细胞迁移,从而发挥促进创面愈合的作用。结论 SA、LPS可促进角质形成细胞迁移从而促进创面愈合;其机制可能为:SA作用于TLR促进细胞释放MMP-1、MMP-9、抑制TIMP-1表达等因素有关。

Preliminary study on mechanism of active Toll-like receptors on surface of HaCat cells to promote cell migration

Objective To investigate the mechanism of free fatty acids(FFA)induced Toll-like receptors activation on HaCat cell surface to promote cell transport.Methods(1)HaCat cells were cultured.(2)TLR2 and TLR4 were detected by Western blot.(3)Cell transport were detected after the cells were cultured with stearic acid(SA,500 μmol/L)and LPS(500 ng/L).(4)Induced Toll-like receptors on the surface of Hacat cells promoted Hacat cells to secrete MMP-1,MMP-9 and TIMP-1.Results SA and LPS activated Toll-like receptors of HaCat cells to promote the expression of TLR-2 and TLR-4 and cell transport.There was a significant difference in cell transport ratio between control group and trial group.Conclusion SA and LPS can activate Toll-like receptors on the surface of HaCat cells to promote cell transport.The probable mechanism is that SA activates dendritic and endothelial cells through TLR,promotes the release of MMP-1 and MMP-9,and inhibits TIMP-1 expression.