大肠癌miR-195表达变化与临床病理学特征的关系 |
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引用本文: | 余志金,甘爱华,张淑英,许岸高,李丙生,陈惠新,彭晓峰,徐永成.大肠癌miR-195表达变化与临床病理学特征的关系[J].中国医师进修杂志,2014,37(28):35-37. |
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作者姓名: | 余志金 甘爱华 张淑英 许岸高 李丙生 陈惠新 彭晓峰 徐永成 |
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作者单位: | 1. 广东省惠州市中心人民医院消化内科,516001 2. 广东省惠州市第一人民医院消化内科 3. 516003,广东省惠州市医学研究所 4. 广东省惠州市中心人民医院消化内科 5. 广东省惠州市第一人民医院病理科 |
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基金项目: | 广东省自然科学基金,广东省惠州市科技计划 |
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摘 要: | 目的 探讨大肠癌miR-195表达变化与临床病理学特征的关系.方法 采用实时荧光定量PCR技术检测miR-195在50例大肠癌患者癌组织及对应癌旁正常组织(距离癌组织>5 cm)中的表达水平,分析其表达情况与临床病理因素的相关性.结果 50例大肠癌患者中共检出miR-195 CpG岛甲基子表型阳性者29例,阳性率为58%(29/50).50例大肠癌患者中有34例出现miR-195在大肠癌组织中的表达较癌旁正常组织下调,即T/N<2.miR-195在大肠癌组织中及癌旁正常组织中的表达水平分别为0.837±1.145和2.236±2.468,差异有统计学意义(P<0.05).miR-195在大肠癌组织中的低表达与患者的年龄、性别、大肠癌部位、浸润深度无关(P>0.05),与患者淋巴结转移、TNM分期有关(P<0.05),在发生淋巴结转移或TNM分期晚的患者中表达明显下调.结论 miR-195在大肠癌组织中的表达呈普遍低表达的趋势,miR-195可能在大肠癌中扮演抑癌基因的角色.miR-195启动子区高甲基化可能是导致miR-195在大肠癌组织中表达下调及参与大肠癌发生发展的原因之一.
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关 键 词: | 结直肠肿瘤 甲基化 |
The relationship between miR-195 expression and clinical pathological characteristics of colorectal cancer |
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Institution: | Yu Zhijin Gan Aihua Zhang Shuying,Xu Angao,Li Bingsheng(Guangdong Province Huizhou City Institute of Medical Research,Guangdong Huizhou 516003, China) |
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Abstract: | Objective To explore the relationship between miR-195 expression and clinical pathological characteristics of colorectal cancer.Methods The expression of miR-195 in 50 cases of colorectal cancer tissue and adjacent non-tumor mucosa (5 cm away from the cancer tissue) was detected by real time PCR.The relationship between miR-195 expression and clinical pathological characteristics of colorectal cancer was analyzed.Results miR-195 CpG island methylate phenotype was positive in 58% (29/50) of patients with colorectal cancer.The expression level of miR-195 in 34 patients colorectal cancer tissue was lower than that in adjacent non-tumor mucosa,that was T/N < 2.The expression level of miR-195 in carcinoma tissue was 0.837 ± 1.145,comparing with adjacent non-tumor mucosa (2.236 ± 2.468),showing significant difference (P < 0.05).Down-regulated expression of miR-195 in carcinoma tissue was independent of age,gender,site(P > 0.05),and related to lymphnode metastasis and TNM stage(P < 0.05).Patients with lymph node metastasis and advanced TNM stage showed significantly reduced expression of miR-195.Conclusions The tendency of common low expression level of miR-195 in human colorectal cancer.MiR-195 maybe play as suppressor gene in human colon carcinoma.Hypermethylation of miR-195 promoter may be one of reasons leading to down-regulation of miR-195 in colorectal cancer tissues and participated in the occurrence and development of human colon carcinoma. |
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Keywords: | Colorectal neoplasms Methylation |
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